Simultaneous saccharification and fermentation of cellulose in ionic liquid for efficient production of α-ketoglutaric acid by Yarrowia lipolytica

Applied Microbiology and Biotechnology
Seunghyun RyuCong T Trinh

Abstract

Ionic liquids (ILs) are benign solvents that are highly effective for biomass pretreatment. However, their applications for scale-up biorefinery are limited due to multiple expensive IL recovery and separation steps that are required. To overcome this limitation, it is very critical to develop a compatible enzymatic and microbial biocatalyst system to carry the simultaneous saccharification and fermentation in IL environments (SSF-IL). While enzymatic biocatalysts have been demonstrated to be compatible with various IL environments, it is challenging to develop microbial biocatalysts that can thrive and perform efficient biotransformation under the same conditions (pH and temperature). In this study, we harnessed the robust metabolism of Yarrowia lipolytica as a microbial platform highly compatible with the IL environments such as 1-ethyl-3-methylimidazolium acetate ([EMIM][OAc]). We optimized the enzymatic and microbial biocatalyst system using commercial cellulases and demonstrated the capability of Y. lipolytica to convert cellulose into high-value organics such as α-ketoglutaric acid (KGA) in the SSF-IL process at relatively low temperature 28 °C and high pH 6.3. We showed that SSF-IL not only enhanced the enzymatic sacchar...Continue Reading

References

Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lee R LyndIsak S Pretorius
Aug 29, 2003·Current Opinion in Biotechnology·Seongsoon Park, Romas J Kazlauskas
Dec 14, 2004·Bioresource Technology·Nathan MosierMichael Ladisch
Feb 10, 2007·Science·Gregory Stephanopoulos
Feb 10, 2007·Science·Michael E HimmelThomas D Foust
Feb 15, 2007·FEMS Yeast Research·Martina TurkNina Gunde-Cimerman
Jun 15, 2007·Chemical Reviews·Fred van Rantwijk, Roger A Sheldon
Apr 22, 2008·Applied and Environmental Microbiology·Cong T TrinhFriedrich Srienc
Dec 19, 2008·Biotechnology and Bioengineering·Sang Hyun LeeJonathan S Dordick
Sep 2, 2009·Progress in Lipid Research·Athanasios BeopoulosJean-Marc Nicaud
Oct 27, 2009·Water Research·Thi Phuong Thuy PhamYeoung-Sang Yun
Jul 20, 2010·Letters in Applied Microbiology·Jingwen ZhouJian Chen
Dec 31, 2010·Biotechnology and Bioengineering·Ying WangNicole Labbé
Jan 20, 2011·Biotechnology and Bioengineering·Kierston ShillHarvey W Blanch
Feb 22, 2011·Biotechnology and Bioengineering·Mauricio Mora-PaleJonathan S Dordick
Jul 1, 2011·The Journal of Microbiology·Jia-Hung WangShu-Hsien Tsai
Oct 4, 2011·Applied Microbiology and Biotechnology·Christina OttoGerold Barth
Jan 1, 2011·Annual Review of Chemical and Biomolecular Engineering·Shishir P S ChundawatBruce E Dale
May 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jane I KhudyakovMichael P Thelen
Jun 19, 2012·Applied Microbiology and Biotechnology·Svetlana V KamzolovaIgor G Morgunov
Aug 17, 2012·Nature·Pamela P Peralta-YahyaJay D Keasling
Oct 20, 2012·Sub-cellular Biochemistry·Andreas AurichAthanassios Giannis
Nov 6, 2012·International Journal of Pharmaceutics·Dorota DoblerFrank Runkel
Jan 30, 2013·Biotechnology for Biofuels·Patanjali VaranasiSeema Singh
Feb 26, 2013·Applied Microbiology and Biotechnology·Svetlana V Kamzolova, Igor G Morgunov
Mar 16, 2013·Critical Reviews in Microbiology·Marizeth GroenewaldMarkus Wyss
Aug 21, 2013·Applied Microbiology and Biotechnology·Igor G MorgunovVladimir A Samoilenko
Mar 29, 2014·Nature Communications·Thomas L RueggMichael P Thelen

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Citations

Dec 5, 2015·ACS Synthetic Biology·Murtaza Shabbir HussainMark Blenner
Nov 1, 2016·Applied Microbiology and Biotechnology·Hongwei GuoGuo Chen
Dec 25, 2016·Applied Microbiology and Biotechnology·Yujin CaoHuizhou Liu
Jul 27, 2017·Chemical Reviews·Toshiyuki Itoh
Dec 20, 2015·Applied and Environmental Microbiology·Seunghyun RyuCong T Trinh
Apr 25, 2020·Microorganisms·Patrick FickersCarol Sze Ki Lin
Feb 7, 2020·Microbial Cell Factories·Maureen HamiltonVasiliki Tsakraklides
Jun 12, 2018·Frontiers in Microbiology·Michael SpagnuoloMark Blenner
Dec 1, 2019·Microbial Cell Factories·Vijaydev GanesanMark Blenner
Aug 13, 2017·Applied Microbiology and Biotechnology·Weng Chan VongShao-Quan Liu
Mar 20, 2019·Metabolic Engineering·Caleb WalkerCong T Trinh

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