Simultaneous selected reaction monitoring, MS/MS and MS3 quantitation for the analysis of pharmaceutical compounds in human plasma using chip-based infusion

Rapid Communications in Mass Spectrometry : RCM
Luc Alexis LeutholdGérard Hopfgartner

Abstract

An assay method with mass spectrometric detection was developed for the quantitative analysis of a pharmaceutical compound and its major metabolite in human plasma using chip-based infusion. Liquid-liquid extraction sample preparation was found to be essential to minimize matrix suppression and to achieve a limit of quantitation (LOQ) of 2.5 ng/mL using a 100 microL plasma aliquot. The potential for simultaneous quantitation in selected reaction monitoring (SRM), tandem mass spectrometry (MS/MS) (enhanced product ion), and MS(3) was investigated and found to be very beneficial in improving assay selectivity. A novel concept for monitoring quantitative assay performance using a SRM/MS(3) ratio is proposed.

Citations

Oct 19, 2011·Analytical and Bioanalytical Chemistry·Emmanuel VaresioGérard Hopfgartner
Jun 25, 2014·Bioanalysis·Steve UngerEdward Wells
Jun 22, 2005·Expert Review of Proteomics·Sheng Zhang, Colleen K Van Pelt
Dec 1, 2004·Rapid Communications in Mass Spectrometry : RCM·Enaksha R WickremsinheAjai K Chaudhary
Jul 29, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Michel WagnerGérard Hopfgartner
Nov 7, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xin LuGuowang Xu
Sep 10, 2005·Electrophoresis·Alina D ZamfirJasna Peter-Katalinić
Nov 9, 2004·Journal of Mass Spectrometry : JMS
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Mar 6, 2009·Rapid Communications in Mass Spectrometry : RCM·Nanyan Rena ZhangWilliam Bart Emary
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Apr 23, 2011·Analytical Chemistry·Tiffany PortaGérard Hopfgartner
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Jan 13, 2010·European Journal of Mass Spectrometry·Luc Alexis LeutholdGérard Hopfgartner
Oct 23, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tomasz ŚniegockiAndrzej Posyniak

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