Simultaneous Sensing of Multiple Cancer Biomarkers by a Single DNA Nanoprobe in a Nanopore

Analytical Chemistry
Bingyuan GuoHai-Chen Wu

Abstract

Both vascular endothelial growth factor (VEGF) and matrix metallopeptidase-9 (MMP-9) are key biomarkers in tumor angiogenesis. Determination of the overexpression of the two biomarkers would provide valuable information on the progression of tumor growth and metastasis, but their simultaneous quantification by a single probe is unprecedented. Here, we develop a triplex DNA-based nanoprobe for simultaneously quantifying VEGF and MMP-9 using an α-hemolysin nanopore. A DNA aptamer is used as the triplex molecular beacon (tMB) loop to bind VEGF, and a stem-forming oligonucleotide modified with a short peptide is used to recognize MMP-9. The sequential presence of VEGF and MMP-9 could also be identified by different patterns of current events. Besides, the characteristic current events generated by the DNA probe possess pH-dependent patterns that can be used to reflect the environmental pH. Success in the construction of such DNA nanoprobes will greatly facilitate the investigation of the mechanisms of different tumor angiogenesis processes and provide a useful approach for cancer diagnosis.

References

Jan 3, 1991·The New England Journal of Medicine·N WeidnerJ Folkman
Nov 18, 1971·The New England Journal of Medicine·J Folkman
Mar 24, 1998·Annual Review of Medicine·B R Zetter
Oct 12, 2000·Nature Cell Biology·G BergersD Hanahan
Sep 15, 2001·Nature·H Bayley, P S Cremer
Feb 28, 2002·Journal of the American Chemical Society·Xingguo LiangMakoto Komiyama
May 7, 2002·Nature Reviews. Cancer·Mikala Egeblad, Zena Werb
Jul 12, 2005·Annual Review of Biomedical Engineering·Robert J Gillies, David L Morse
Oct 13, 2006·Journal of the American Chemical Society·Preston T SneeDaniel G Nocera
Nov 18, 2006·Cell·Gaorav P Gupta, Joan Massagué
May 31, 2007·Angewandte Chemie·Tom N GrossmannOliver Seitz
Mar 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Siân JonesSanford D Markowitz
May 9, 2008·The New England Journal of Medicine·Robert S Kerbel
Jan 17, 2009·Journal of the American Chemical Society·Junyan HanKevin Burgess
Jul 28, 2010·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Xiaomeng ZhangRobert J Gillies
Jul 29, 2011·Analytical Chemistry·Jing ZhengWeihong Tan
Mar 30, 2013·Science·Bert VogelsteinKenneth W Kinzler
Sep 28, 2016·Angewandte Chemie·Lei Liu, Hai-Chen Wu
Apr 27, 2017·Angewandte Chemie·Yuwei HuItamar Willner
Sep 12, 2019·Journal of the American Chemical Society·Yi-Lun Ying, Yi-Tao Long

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