Simultaneous separate detection of low angle and large angle light scattering in an arc lamp-based flow cytometer

Cytometry
H B Steen

Abstract

A device is described for simultaneous separate detection of the light scattering of cells at low and large scattering angles in an arc lamp-based flow cytometer with epi-illumination through an oil immersion microscope objective. Light scattering was measured in a dark field configuration that allows separate detection of light scattering greater than 2 degrees and 15 degrees, respectively. Dual parameter light scattering histograms of a blood cell suspension containing various types of leukocytes were closely similar to that obtained with a commercial laser-based instrument with light scattering detection at forward and right angles. The sensitivity of the device was sufficient to measure polystyrene particles with 0.25-micron diameter. A potential application may be differentiation of bacteria.

References

Apr 27, 1979·Science·H B Steen, T Lindmo
Jan 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M R LokenL A Herzenberg
Feb 1, 1983·The Histochemical Journal·H B Steen

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Citations

Jan 14, 1998·International Journal of Cancer. Journal International Du Cancer·J MoanK Berg
Mar 14, 2002·Cytometry·Harald B Steen, Trond Stokke
Sep 23, 2000·Journal of Microbiological Methods·H B Steen
Feb 7, 2001·Clinical Microbiology Reviews·A Alvarez-BarrientosM Sánchez-Pérez
Jan 30, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Harald B Steen
Dec 17, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Gérald GrégoriJ Paul Robinson
Jul 12, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Cherry GreinerIrene Georgakoudi
Jan 15, 1989·International Journal of Cancer. Journal International Du Cancer·H HolteR Ohlsson
Apr 13, 2019·Annual Review of Analytical Chemistry·Hong LianXiaomei Yan
Sep 5, 2008·Current Protocols in Cytometry·H B Steen
Sep 5, 2008·Current Protocols in Cytometry·J Paul Robinson
Jul 2, 2015·Current Protocols in Cytometry·John P Nolan
Jul 25, 2018·Current Protocols in Cytometry·J Paul Robinson
Feb 6, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Leonie de RondFrank A W Coumans

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