Simultaneous Separation and Quantification of Vitamins by Microemulsion Liquid Chromatography.

Journal of Chromatographic Science
Tongtong XuNing Li

Abstract

Microemulsion eluents have been found to have excellent potential uses in high-performance liquid chromatography (HPLC). Here, a novel, environmentally benign and simple method using concentration/flow-rate double-gradient elution using a microemulsion eluent was used to separate water- and fat-soluble vitamins simultaneously and rapidly. Preliminary screening experiments were performed to determine the optimum column type, surfactant concentration, co-surfactant to surfactant ratio, oil, mobile phase pH and microemulsion concentration. The resolution and analysis time were simultaneously optimized using concentration/flow-rate double-gradient elution. The optimized method simultaneously separated water- and fat-soluble vitamins using a Venusil ASB C8 column and a combination of isocratic and linear gradient elution modes using a microemulsion mobile phase (solvent A) consisting of 3.5% (w/w) sodium dodecyl sulfate, 10.5% (w/w) n-butanol, 0.8% (w/w) n-octanol and 85.2% (w/w) water and water (solvent B) at pH 2.50. The optimum detection wavelength was 283 nm. The method was validated and used to analyze a solid pharmaceutical sample.

References

Jan 11, 2005·Journal of Separation Science·Simon M Bryant, Kevin D Altria
May 20, 2008·Analytica Chimica Acta·María Vilasoa-MartínezAna Rodríguez-Bernaldo de Quirós
Dec 15, 2010·Talanta·Marco CiuluGavino Sanna
Oct 22, 2011·Journal of Chromatography. a·Helena Maria Pinheiro-Sant'anaSebastião César Cardoso Brandão
Sep 23, 2014·Journal of Pharmaceutical and Biomedical Analysis·Ilona OlędzkaTomasz Bączek
Mar 1, 2016·The Journal of Steroid Biochemistry and Molecular Biology·Ali A AlbahraniRonda F Greaves

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Citations

Aug 16, 2021·Magnetic Resonance Imaging·Delphine HerreraChristine Dalmazzone

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