PMID: 9432040Jan 31, 1998Paper

Simultaneous SIL-TAL1 RT-PCR detection of all tal(d) deletions and identification of novel tal(d) variants

British Journal of Haematology
E DelabesseE A Macintyre

Abstract

Site-specific deletions of the 5' part of the TAL1 gene (tal(d)) are among the most frequent non-random genetic abnormalities in T-cell acute lymphoblastic leukaemia (T-ALL). They are usually detected by PCR from DNA with several primer pairs or by Southern blot analysis. Since tal(d) lead to expression of a SIL-TAL1 fusion transcript, irrespective of the genomic breakpoint, we have used a single monoplex RT-PCR reaction to screen 55 T-ALL patients at diagnosis. SIL-TAL1 transcripts were demonstrated in 12 (22%) cases, including 7/27 (26%) children <15 years of age, 2/8 (25%) adolescents and 2/17 (12%) adults aged >20 years. SIL-TAL1 RT-PCR was preferrable to tal(d) DNA PCR since it allowed the simultaneous detection of tal(d), tal(d2) and two previously undescribed tal(d) variants. SIL-TAL1 RT-PCR screening should therefore increase the detection rate of tal(d) by approximately 15-20%, with an at least comparable sensitivity to tal(d) genomic PCR, and represents a more practical and economic alternative to multiple DNA PCRs or Southern blotting when incorporated into molecular screening for multiple transcripts at diagnosis.

Citations

Dec 3, 1999·The International Journal of Biochemistry & Cell Biology·L M BartonA R Green
Aug 8, 1998·British Journal of Haematology·E DelabesseE A Macintyre
Jun 30, 2009·Leukemia & Lymphoma·Marcela Braga MansurMaria S Pombo-de-Oliveira
Nov 25, 2003·British Journal of Haematology·M T RubioJ P Vernant
May 4, 2002·Best Practice & Research. Clinical Haematology·Giovanni CazzanigaAndrea Biondi
Nov 28, 2002·Leukemia·L MauvieuxM Lessard
Feb 16, 2012·Genes, Chromosomes & Cancer·Masahiro Onozawa, Peter D Aplan
Feb 28, 2014·Asia-Pacific Journal of Clinical Oncology·Heba Moussa, Iman Sidhom
Aug 13, 2015·Asia-Pacific Journal of Clinical Oncology·Anita ChopraRajive Kumar
Oct 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Janet M MurrayBarry A Finette
Feb 5, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Frederic BaleydierElizabeth Macintyre

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