Simultaneous solid phase extraction, derivatization, and gas chromatographic mass spectrometric quantification of thromboxane and prostacyclin metabolites, prostaglandins, and isoprostanes in urine

Analytical Chemistry
J WübertR L Lorenz

Abstract

The current analytical methods for the various prostanoids require a separate and extended sample workup, derivatization, and gas chromatographic/mass spectrometric detection of each compound. Therefore, we developed and validated a rapid method for the common purification, derivatization, and GC/MS determination of 11-dehydrothromboxane B2, 2,3-dinor-6-keto-PGF1a, PGF2A, PGE2, PGD2, and isoprostanes in urine. A single reversed-phase solid-phase extraction step and modified reaction conditions yielded excellent sample purification at high recoveries and efficient derivatization for all compounds in one vial. The method allows, for the first time, the simultaneous quantification of these index metabolites of systemic thromboxane and prostacyclin synthesis, renal prostaglandin formation, and nonenzymatic in vivo lipid peroxidation in a single GC/MS run with high sensitivity and precision.

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Citations

Jun 22, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hiroki TsukamotoMichinao Mizugaki
Jan 8, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Edzard Schwedhelm, Rainer H Böger
May 15, 2007·Prostaglandins & Other Lipid Mediators·Toni N WelshTamas Zakar
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Jan 6, 2004·Free Radical Research·J W M Yuen, I F F Benzie
Nov 17, 2006·The Journal of Biological Chemistry·Huiyong YinJason D Morrow
Sep 13, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andreas NievergeltJürg Gertsch
Oct 8, 2005·The American Journal of Clinical Nutrition·Henry J ThompsonWeiqin Jiang

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