Simultaneous spatiotemporal tracking and oxygen sensing of transient implants in vivo using hot-spot MRI and machine learning

Proceedings of the National Academy of Sciences of the United States of America
Virginia SpanoudakiDaniel G Anderson

Abstract

A varying oxygen environment is known to affect cellular function in disease as well as activity of various therapeutics. For transient structures, whether they are unconstrained therapeutic transplants, migrating cells during tumor metastasis, or cell populations induced by an immunological response, the role of oxygen in their fate and function is known to be pivotal albeit not well understood in vivo. To address such a challenge in the case of generation of a bioartificial pancreas, we have combined fluorine magnetic resonance imaging and unsupervised machine learning to monitor over time the spatial arrangement and the oxygen content of implants encapsulating pancreatic islets that are unconstrained in the intraperitoneal (IP) space of healthy and diabetic mice. Statistically significant trends in the postimplantation temporal dependence of oxygen content between aggregates of 0.5-mm or 1.5-mm alginate microcapsules were identified in vivo by looking at their dispersity as well as arrangement in clusters of different size and estimating oxygen content on a pixel-by-pixel basis from thousands of 2D images. Ultimately, we found that this dependence is stronger for decreased implant capsule size consistent with their tendency ...Continue Reading

References

Aug 1, 1976·Journal of Applied Physiology·M E TowellS P Bessman
Jun 1, 1974·American Journal of Surgery·J KlossnerJ Niinikoski
Jul 1, 1994·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·B J Dardzinski, C H Sotak
Mar 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·P S Hees, C H Sotak
Nov 26, 1999·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·U NöthJ Lutz
Aug 28, 2001·Journal of Biomedical Materials Research·A KingA Andersson
Jul 26, 2005·Nature Biotechnology·Eric T AhrensPenelope A Morel
Nov 23, 2006·Journal of Microencapsulation·Marcus D DarrabieEmmanuel C Opara
May 23, 2007·Tissue Engineering·Jos MaldaZee Upton
Jul 1, 2008·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Kenneth A KrohnRalph P Mason
Nov 19, 2009·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Jelena M Janjic, Eric T Ahrens
Aug 20, 2010·Cell Transplantation·Jamal DaoudMaryam Tabrizian
Sep 16, 2010·NMR in Biomedicine·Jesús Ruiz-CabelloJeff W M Bulte
Nov 12, 2010·Tissue Engineering. Part C, Methods·Amy S JohnsonClark K Colton
Apr 14, 2011·Tissue Engineering. Part C, Methods·Fernie Goh, Athanassios Sambanis
Feb 22, 2012·Journal of Materials Science. Materials in Medicine·Monica L MoyaEric M Brey
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Eileen PedrazaCherie L Stabler
Jul 14, 2012·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Sean Carlin, John L Humm
Nov 20, 2012·Journal of the American Chemical Society·Shodai TakahashiKenjiro Hanaoka
Jan 29, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·L LemaireJ P Benoit
Jun 19, 2013·Biomaterials·Alyssa A AppelEric M Brey
Dec 18, 2013·Neoplasia : an International Journal for Oncology Research·Yunzhou ShiRichard A D Carano
Apr 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Vincent H LiuMichael J Cima
Sep 23, 2014·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Eric T AhrensClaudiu Schirda
Apr 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Alexandra BerdichevskiDror Seliktar
Apr 22, 2015·Nature Biotechnology·Andrew R PepperA M James Shapiro
May 29, 2015·Nature·Yann LeCunGeoffrey Hinton
Apr 29, 2016·Science·Erinn B Rankin, Amato J Giaccia
Dec 23, 2016·Nature Reviews. Drug Discovery·Tejal Desai, Lonnie D Shea

❮ Previous
Next ❯

Citations

Mar 3, 2020·Journal of Diabetes Science and Technology·Sydney C WigginsCherie L Stabler
May 30, 2020·Chemistry : a European Journal·Nicholas G TaylorFrank A Leibfarth
Mar 20, 2021·Frontiers in Endocrinology·Dian R Arifin, Jeff W M Bulte
Jun 12, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tania B Lopez-MendezRosa Maria Hernandez
Jul 5, 2019·Biomacromolecules·Nureddin AshammakhiAli Khademhosseini

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.