PMID: 16512420Mar 4, 2006Paper

Simultaneous spectrofluorometric determination of piroxicam and pyridoxine using generalized rank annihilation method

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
H AbdollahiAbdolhossein Naseri

Abstract

The application of generalized rank annihilation method (GRAM) to the analysis of fluorescence excitation-emission matrices of mixtures of piroxicam and pyridoxine is described. The input of GRAM consists of two bilinear data matrices, i.e. one for unknown and one for the calibration sample. The excitation wavelength range was from 290 to 340 nm and the emission was recorded from 370 to 560 nm. Piroxicam and pyridoxine were determined in the concentration ranges 0.33 - 4.00 microg ml(-1) and 0.66 - 8.00 microg ml(-1), respectively. To check the accuracy of the proposed method, several binary synthetic mixtures and one real sample were analyzed successfully. No matrix effect was observed in mixture analysis, so a single external calibration sample was used for each analyte. The ability of GRAM to quantify the studied compounds and the comparability of GRAM results were evaluated by comparing them with those of PLS regression as a standard first-order calibration.

References

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Citations

Oct 16, 2007·Journal of Biochemical and Biophysical Methods·Mohammad-Hossein SorouraddinMohammad-Reza Rashidi
Feb 1, 2011·Analytical Methods : Advancing Methods and Applications·Abdolhossein Naseri, Hamid Abdollahi

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