Simultaneous stacking of cationic and anionic compounds in single run capillary zone electrophoresis by two-end field amplified sample injection

Journal of Chromatography. a
Xiaoling HouJiyou Zhang

Abstract

In order to extend the application of field amplified sample injection (FASI) in high throughput analysis, a convenient and simple procedure, namely two-end field amplified sample injection (TE-FASI), was developed for the simultaneous stacking of cationic and anionic compounds in a single run capillary zone electrophoresis (CZE). Following the capillary-filling with a buffer of high conductivity, water plug was loaded into each end of the capillary; and two high-field strength zones were generated at both heads of the column when high voltage was applied. Therefore, under suppressed EOF cations and anions can be selectively FASI stacked at anode and cathode head, respectively. After separation, the stacked anions and cations are detected by a common detector placed in the center of the capillary. Under the optimized conditions, the limits of detection for the model cationic (matrine and oxymatrine) and anionic (5-sulfosalicylic acid) compounds were determined as 0.2, 0.2 and 0.06 ng/mL, respectively. Compared with non-stacking conditions, the sensitivities of these compounds were enhanced 1003-, 1330- and 1380-fold, respectively. The results of reproducibility, linearity and real sample analysis show that the proposed procedur...Continue Reading

Citations

Mar 22, 2013·Journal of Chromatographic Science·Zhaoxiang ZhangYing Liu
Aug 12, 2014·Analytical and Bioanalytical Chemistry·Dong-Sheng LianBing-Ling Li
Feb 22, 2012·Oral Oncology·Jingyi Liu, Yixiang Duan
May 7, 2015·Biomicrofluidics·An Eng LimYee Cheong Lam
Nov 12, 2013·Journal of Chromatography. a·Fumihiko Kitagawa, Koji Otsuka
Nov 20, 2012·Electrophoresis·Andrea ŠlampováPetr Boček
Apr 23, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Fumihiko KitagawaIsoshi Nukatsuka
Dec 6, 2011·Electrophoresis·Xiao-jia ChenShao-Ping Li
Nov 20, 2016·Electrophoresis·Donna M Blackney, Joe P Foley
Dec 14, 2011·Analytical Chemistry·Matthew GeigerMichael T Bowser

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