PMID: 8950599Oct 1, 1996Paper

Simultaneous ultrastructural identification of growth hormone and its messenger ribonucleic acid using combined immunohistochemistry and non-radioisotopic in situ hybridization: a technical note

The Histochemical Journal
Akira MatsunoT Nagashima

Abstract

The present electron microscopical study is concerned with the simultaneous visualization of messenger ribonucleic acid (mRNA) and its encoded protein in the same specimen. Pre-embedding electron microscopical in situ hybridization (EM-ISH) on rat pituitary gland tissue localized growth hormone mRNA in the polysomes of the rough endoplasmic reticulum, and subsequent postembedding immunolabelling using protein A-colloidal gold particles identified growth hormone mainly in the secretory granules. We believe that our report provides the first simultaneous ultrastructural identification of mRNA and its encoded protein using combined pre-embedding EM-ISH and immunohistochemistry. In this method, the signals for mRNA were localized specifically as highly electron dense products on the polysomes of the endoplasmic reticulum, and those for its encoded protein were recognized as gold particles both in the cisternae of the reticulum and in the secretory granules. Our ultrastructural double labelling method for mRNA and protein may provide a tool to find important clues for elucidating the intracellular correlation of mRNA translation and secretion of translated protein, because of its high resolution, good morphological preservation, and...Continue Reading

References

Jul 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D Le GuellecG Morel
Jun 1, 1991·Pathology, Research and Practice·R V LloydW F Chandler
Jan 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D Le GuellecP Y Desprez
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G F JirikowskiF E Bloom
Jan 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R A WolberH F Maassab
Aug 1, 1995·The Journal of Clinical Endocrinology and Metabolism·N SannoR Y Osamura
Jun 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D EggerK Bienz

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Citations

Oct 23, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G J Nuovo
Jun 17, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R Y OsamuraA Matsuno
Jul 5, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Akira MatsunoR Yoshiyuki Osamura
May 5, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Akira MatsunoR Yoshiyuki Osamura

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