Simvastatin enhances irinotecan-induced apoptosis in prostate cancer via inhibition of MCL-1

Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society
Mohammad A Y AlqudahNizar Mhaidat

Abstract

Prostate cancer is one of the most common malignant tumors around the world. Hyperlipidemia is considered as one of the most important risk factors for the development of prostate cancer. Simvastatin is widely used for the treatment of hyperlipidemia and was previously shown to induce apoptosis in several cancer types including lung, colon, pancreas, breast, and prostate cancer. In this study we aimed to explore the potential role of simvastatin in enhancing irinotecan-induced apoptosis in prostate cancer cells. In addition, the underlying molecular mechanisms driving this potential effect of simvastatin were also explored. PC3 cells were treated with simvastatin, irinotecan or combination. Cell viability was assessed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) colorimetric assay. Flow cytometry technique was used to analyze apoptosis and cell cycle progression. Western blot was used for detection of protein expression. Results showed that simvastatin has a significant anti-proliferative activity on PC3 cells. Combined treatment of simvastatin with irinotecan exhibited a significant inhibition of PC3 cell growth compared to each treatment alone. Flow cytometry analysis showed that PC3 cell treatmen...Continue Reading

Citations

Jun 15, 2019·Histochemistry and Cell Biology·Dongmei SuYunjun Zhou
Dec 5, 2020·Medicinal Research Reviews·Henrique J CardosoSílvia Socorro
Aug 20, 2020·Seminars in Cancer Biology·Amir R AfshariAmirhossein Sahebkar
Jul 26, 2021·Journal of Experimental & Clinical Cancer Research : CR·Wen JiangXin-Yang He

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Methods Mentioned

BETA
flow cytometry
Electrophoresis
FACS

Software Mentioned

Graphpad Prism

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