Sinapic Acid Inhibits IL-1β-Induced Apoptosis and Catabolism in Nucleus Pulposus Cells and Ameliorates Intervertebral Disk Degeneration

Journal of Inflammation Research
Jin-Feng HuangAimin Wu

Abstract

Low back pain (LBP) is a very common condition and leads to serious pain, disability, and price tag all over the world. Intervertebral disk degeneration (IDD) is one of the major reasons that contributed to LBP. The levels of interleukin 1 beta (IL-1β) increase significantly in degenerative disks. IL-1β also accelerates IDD. Sinapic acid (SA) has the effect of anti-inflammatory, antioxidant and antimicrobial. However, the effect of SA on IDD has never been studied. Therefore, the aim of this study was to figure out whether SA has protective effect on nucleus pulposus (NP) cells and further explore the possible underlying mechanism. The nucleus pulposus (NP) tissues of rats were collected and cultured into NP cells. The NP cells were stimulated by IL-1β and treated with SA. In vitro treatment effects were evaluated by ELISA, Western blot assay, immunofluorescence, TUNEL method and real-time PCR. We conducted percutaneous needle puncture in the rat tail to build intervertebral disk degeneration model and treated rats with SA. In vivo treatment effects were evaluated by hematoxylin and eosin (HE) and safranin O (SO) staining and magnetic resonance imaging (MRI) method. Our results showed that SA not only inhibited apoptosis but al...Continue Reading

References

Sep 2, 1999·Lancet·G B Andersson
Mar 9, 2000·Spine·K LuomaA Lamminen
Aug 19, 2003·Spine·Gabriele K LutzUlrich Schultz-Venrath
Jul 1, 2005·Arthritis Research & Therapy·Christine Lyn Le MaitreJudith Alison Hoyland
Sep 14, 2006·Annual Review of Pharmacology and Toxicology·Thomas W KenslerShyam Biswal
Sep 18, 2007·Ageing Research Reviews·Chang-Qing ZhaoLi-Yang Dai
Aug 7, 2010·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jae Man LeeDong Eun Shin
Jun 16, 2012·Osteoarthritis and Cartilage·J ScheeleS M A Bierma-Zeinstra
Mar 21, 2013·Apoptosis : an International Journal on Programmed Cell Death·Fan DingLi-ming Xiong
Jul 12, 2013·JAMA : the Journal of the American Medical Association·Christopher J L MurrayUNKNOWN U.S. Burden of Disease Collaborators
Oct 30, 2013·Nature Reviews. Rheumatology·Makarand V Risbud, Irving M Shapiro
Apr 25, 2014·The Journal of Biological Chemistry·Antonio CuadradoIsabel Lastres-Becker
Sep 2, 2014·Advanced Drug Delivery Reviews·Gianluca FontanaAbhay Pandit
Apr 2, 2015·Osteoarthritis and Cartilage·P-P A VergroesenT H Smit
Nov 10, 2015·Biochemical Society Transactions·Joanna D WardynChristopher M Sanderson
Apr 14, 2016·Oxidative Medicine and Cellular Longevity·Chunye Chen
Aug 16, 2016·Seminars in Arthritis and Rheumatism·Edward YelinToby King
Oct 18, 2016·Lancet·Chris MaherRachelle Buchbinder
Mar 28, 2017·The Spine Journal : Official Journal of the North American Spine Society·Cun-Xin ZhangDe-Chun Wang
Jul 8, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mushtaq Ahmad Ansari
Feb 28, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Hao YangXiangrui Wang
Apr 2, 2018·Matrix Biology : Journal of the International Society for Matrix Biology·Hyowon ChoiMakarand V Risbud
Apr 11, 2018·JAMA : the Journal of the American Medical Association·UNKNOWN US Burden of Disease CollaboratorsChristopher J L Murray
Mar 9, 2019·Rheumatology International·Francis FatoyeIsaac Odeyemi

❮ Previous
Next ❯

Citations

Mar 8, 2021·Biochemical and Biophysical Research Communications·Wang DingXiaofan Yin
May 11, 2021·Oxidative Medicine and Cellular Longevity·Xi LuoJiangang Shi

❮ Previous
Next ❯

Methods Mentioned

BETA
Protein Assay
electrophoresis
ELISA
PCR
nuclear translocation

Software Mentioned

SPSS
Image J
Image Lab

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis