Sindbis viral-mediated expression of Ca2+-permeable AMPA receptors at hippocampal CA1 synapses and induction of NMDA receptor-independent long-term potentiation

The European Journal of Neuroscience
T OkadaS Ozawa

Abstract

Gene manipulation in order to artificially express a particular gene in neurons in the central nervous system is a powerful tool for the analysis of brain function. Sindbis viral vectors have been developed to express high levels of foreign genes in postmitotic brain neurons with little transfection of glial cells. In this study, we expressed the gene encoding the unedited GluR2 (GluR-B) subunit of the AMPA-type glutamate receptor that forms inwardly rectifying and Ca2+-permeable channels, in rat CA1 hippocampal neurons in slice cultures using Sindbis viral vectors. The pyramidal cell layer of the CA1 region was injected with recombinant Sindbis viruses encoding both enhanced green fluorescent protein (GFP) and unedited GluR2. The GFP fluorescence from CA1 neurons could be detected as early as 6 h and reached a maximal level about 48 h postinfection. The inwardly rectifying and Ca2+-permeable AMPA receptors were expressed in most CA1 pyramidal cells expressing GFP. These AMPA receptors expressed by gene transfer were involved in fast excitatory neurotransmission elicited by electrical stimulation of the Schaffer collaterals in the stratum radiatum. Tetanic stimulation of Schaffer collaterals induced NMDA receptor-independent, l...Continue Reading

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Citations

Oct 10, 2002·Brain Research Bulletin·Markus U Ehrengruber
Jan 25, 2003·The European Journal of Neuroscience·Takashi OkadaSeiji Ozawa
Nov 26, 2003·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Takeshi SuzukiShin Kwak
Jul 13, 2004·The European Journal of Neuroscience·Wataru KakegawaSeiji Ozawa
Oct 3, 2009·Journal of Neuroscience Research·Estelle DrobacBertrand Lambolez
Sep 24, 2004·Neurochemistry International·Mattias TranbergMats Sandberg
Dec 15, 2004·Journal of Pharmacological and Toxicological Methods·K H S ArunP Ramarao
Feb 27, 2003·The European Journal of Neuroscience·Wataru KakegawaSeiji Ozawa
Jul 12, 2017·Molecular Psychiatry·N BenamerL Tricoire
Mar 30, 2004·Annals of Neurology·Jennifer L Nargi-AizenmanDiane E Griffin
Apr 27, 2004·Nature Neuroscience·Juha-Pekka HimanenDimitar B Nikolov
Dec 14, 2018·Frontiers in Synaptic Neuroscience·Pojeong ParkGraham L Collingridge

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