Sinensetin enhances adipogenesis and lipolysis by increasing cyclic adenosine monophosphate levels in 3T3-L1 adipocytes

Biological & Pharmaceutical Bulletin
Seong-Il KangSe-Jae Kim

Abstract

Sinensetin is a rare polymethoxylated flavone (PMF) found in certain citrus fruits. In this study, we investigated the effects of sinensetin on lipid metabolism in 3T3-L1 cells. Sinensetin promoted adipogenesis in 3T3-L1 preadipocytes growing in incomplete differentiation medium, which did not contain 3-isobutyl-1-methylxanthine. Sinensetin up-regulated expression of the adipogenic transcription factors peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein (C/EBP) α, and sterol regulatory element-binding protein 1c. It also potentiated expression of C/EBPβ and activation of cAMP-responsive element-binding protein. Sinensetin enhanced activation of protein kinase A and increased intracellular cAMP levels in 3T3-L1 preadipocytes. In mature 3T3-L1 adipocytes, sinensetin stimulated lipolysis via a cAMP pathway. Taken together, these results suggest that sinensetin enhances adipogenesis and lipolysis by increasing cAMP levels in adipocytes.

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Citations

Feb 19, 2019·Bioscience, Biotechnology, and Biochemistry·Eisuke Kato
Oct 30, 2019·BMC Complementary and Alternative Medicine·Jin-A KimGon Sup Kim
Apr 26, 2017·Molecular Medicine Reports·Guang-Chun PiaoHai-Dan Yuan
Feb 26, 2021·Frontiers in Pharmacology·Lee Han JieKhairana Husain
May 28, 2021·International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition·Mahdi Vajdi, Mahdieh Abbasalizad Farhangi
Jul 18, 2021·Journal of Ethnopharmacology·Anuj Kumar BorahJagat Chandra Borah

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