Single- and double-stranded RNA measurements by flow cytometry in solid neoplasms

Cytometry
A K el-NaggarB Barlogie

Abstract

We investigated the ability of single- and double-stranded RNA measurements to discriminate between neoplastic and non-neoplastic solid tissues. Sixty-one solid nonhematopoietic neoplasms, 10 reactive non-neoplastic lesions, and 26 normal tissue samples were the test materials. Single-stranded ribonucleic acid (s-RNA) levels and double-stranded ribonucleic acid (ds-RNA) excess in these specimens were defined in relationship to normal human lymphocytes. The mean s-RNA index in normal, reactive, benign, and diploid and aneuploid malignant tissue samples was 0.90, 1.54, 1.9, 1.2, and 2.2, respectively. For ds-RNA, the mean excess level for normal, reactive, benign, and diploid and aneuploid malignant specimens was 8.5%, 18.5%, 51.0%, 36.0%, and 41.3%, respectively. No statistical differences in s-RNA level were found between non-neoplastic and neoplastic tissue samples. A significant difference in ds-RNA excess was found between non-neoplastic and benign, and diploid and aneuploid malignant neoplastic tissue (P less than 0.001). The specificity of s-RNA level and ds-RNA excess was 94.4% and 100%, and the sensitivity was 29.5% and 67.2%, respectively. Notably, ds-RNA determinations identified 70.0% of the diploid neoplastic samples...Continue Reading

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