Single-allele analysis of transcription kinetics in living mammalian cells.

Nature Methods
Sharon YungerYaron Shav-Tal

Abstract

We generated a system for in vivo visualization and analysis of mammalian mRNA transcriptional kinetics of single alleles in real time, using single-gene integrations. We obtained high-resolution transcription measurements of a single cyclin D1 allele under endogenous or viral promoter control, including quantification of temporal kinetics of transcriptional bursting, promoter firing, nascent mRNA numbers and transcription rates during the cell cycle, and in relation to DNA replication.

References

Oct 6, 1995·The Journal of Biological Chemistry·C AlbaneseR G Pestell
Jan 1, 1997·Annual Review of Biophysics and Biomolecular Structure·M J Saxton, K Jacobson
May 9, 1998·Science·A M FeminoR H Singer
Nov 11, 1998·Molecular Cell·E BertrandR M Long
Jul 13, 2000·Methods in Enzymology·P ChartrandR M Long
Jan 28, 2003·Current Biology : CB·Dahlene FuscoEdouard Bertrand
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·L D MesnerP A Dijkwel
Jun 11, 2004·Biophysical Journal·Brian L SpragueJames G McNally
Jun 19, 2004·Science·Yaron Shav-TalRobert H Singer
May 20, 2005·Current Opinion in Cell Biology·Xavier DarzacqYaron Shav-Tal
Nov 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Diana Y VargasSanjay Tyagi
Dec 20, 2005·Cell·Ido GoldingEdward C Cox
May 23, 2006·Current Biology : CB·Jonathan R ChubbRobert H Singer
Aug 7, 2007·Nature Structural & Molecular Biology·Xavier DarzacqRobert H Singer
Oct 24, 2007·The Journal of Cell Biology·Stéphanie BoireauEdouard Bertrand
Aug 8, 2009·Physical Review Letters·I BronsteinY Garini
Sep 29, 2009·Molecular Cell·Gordon L HagerTom Misteli

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Citations

Jun 12, 2013·Histochemistry and Cell Biology·Sharon YungerYaron Shav-Tal
Sep 24, 2013·Chemical Reviews·Jens Michaelis, Barbara Treutlein
May 22, 2013·Journal of Medicinal Chemistry·Meirav SegalBilha Fischer
May 3, 2011·Nature Genetics·Lok-Hang SoIdo Golding
Jan 18, 2011·Nature Methods·Timothée LionnetRobert H Singer
Jul 10, 2013·Nature Reviews. Genetics·Antoine CoulonDaniel R Larson
Sep 6, 2011·Nature Structural & Molecular Biology·Sandra Bento MartinsMaria Carmo-Fonseca
Apr 5, 2013·Nature Structural & Molecular Biology·Anne HelmrichLaszlo Tora
Apr 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Tetsuya MuramotoJonathan R Chubb
Oct 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Roy D DarLeor S Weinberger
Apr 20, 2011·Cold Spring Harbor Symposia on Quantitative Biology·T LionnetD R Larson
May 19, 2011·Development, Growth & Differentiation·Michelle StevenseTetsuya Muramoto
Dec 7, 2013·Science·Alvaro Sanchez, Ido Golding
Jan 26, 2013·Genome Biology·Alon Kalo, Yaron Shav-Tal
Sep 10, 2011·Journal of Visualized Experiments : JoVE·Yehuda Brody, Yaron Shav-Tal
Jul 6, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jacques P BothmaMichael Levine
Feb 22, 2013·Molecular Biology of the Cell·Alexandre Jose Christino QuaresmaJeffrey A Nickerson
Sep 21, 2013·Annual Review of Genetics·Hojoong Kwak, John T Lis
Sep 10, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Masae OhnoYuichi Taniguchi
Apr 10, 2012·Biochimica Et Biophysica Acta·Diana A StavrevaGordon L Hager
Apr 7, 2012·Biochimica Et Biophysica Acta·Murali Palangat, Daniel R Larson
Mar 1, 2012·Current Opinion in Genetics & Development·Lucas Brandon Edelman, Peter Fraser
Feb 14, 2012·Biochimica Et Biophysica Acta·Sanjeev Shukla, Shalini Oberdoerffer
Oct 4, 2011·Current Opinion in Cell Biology·David M SuterUeli Schibler
Jan 6, 2016·Nucleus·Yaron Shav-Tal, Jan Lammerding
Sep 1, 2014·Current Opinion in Biotechnology·Anna K Rath, Andrea Rentmeister
Mar 5, 2016·Science·Yoav VoichekNaama Barkai
Dec 9, 2014·RNA Biology·Martyna O UrbanekWlodzimierz J Krzyzosiak
Mar 14, 2012·EMBO Reports·Timothée Lionnet, Robert H Singer
Oct 22, 2011·EMBO Reports·Paolo MaiuriAlessandro Marcello
Aug 30, 2012·Molecular Systems Biology·Abhyudai SinghLeor S Weinberger
Nov 19, 2011·EMBO Reports·Danielle Cannon, Jonathan R Chubb
Aug 10, 2013·Journal of Cellular Physiology·Alyshia Newhart, Susan M Janicki
Oct 8, 2015·Nature Reviews. Genetics·Björn Reinius, Rickard Sandberg
Aug 25, 2011·Current Opinion in Genetics & Development·Daniel R Larson
Apr 13, 2013·Nucleus·Jonathan Sheinberger, Yaron Shav-Tal

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