PMID: 2499886Jun 1, 1989Paper

Single amino acid exchanges in the finger domain impair the function of the Drosophila gene Krüppel (Kr)

Proceedings of the National Academy of Sciences of the United States of America
U GaulH Jäckle

Abstract

Mutations in the Drosophila gene Krüppel (Kr) cause deletions of segments in the embryo and also affect Malpighian tubule development. In the hypomorphic Kr alleles that were analyzed, the defects in the segment pattern and the Malpighian tubules are parallel in strength. We have sequenced the DNA of four Kr alleles that show normal spatial and temporal patterns of Kr-encoded protein expression. Three of the four alleles have single base pair mutations that result in a single amino acid change. The exchanges occur in the putative DNA-binding domain of the Kr protein, which is characterized by four repeats of the zinc finger motif. Sequence comparison of the finger motifs helped to define the structural requirements for the folding of the finger domain to some extent. Our data on the Kr mutants support the view that has emerged from the evolutionary analysis.

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Citations

Nov 1, 1991·Mechanisms of Development·T el-Baradi, T Pieler
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·P CarreraH Jäckle
Feb 28, 1991·Biochemical and Biophysical Research Communications·H J Thiesen, B Schröder
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·S A WolfeC O Pabo
Jul 5, 1990·Nature·J D LichtU M Hansen
May 8, 2004·The Journal of Biological Chemistry·Alexey MatyashHerbert Jäckle
Nov 1, 1989·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Detlef WeigelHerbert Jäckle

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