Single amino acid in V2 encoded by TYLCV is responsible for its self-interaction, aggregates and pathogenicity

Scientific Reports
Wenhao ZhaoYongjian Fan

Abstract

The V2 protein encoded by Begomovirus is essential for virus infection and is involved in multiple functions, such as virus movement and suppression of the host defence response. In this study, we reported that V2 encoded by the Tomato yellow leaf curl virus (TYLCV), which is one of the most devastating tomato-infecting begomoviruses, could interact with itself and a S71A mutation of V2 (V2S71A) abolished its self-interaction. Fluorescence results showed that V2 localized primarily in the cytoplasm and around the nucleus. Site-directed mutagenesis V2S71A had the similar subcellular localization, but V2S71A formed fewer large aggregates in the cytoplasm compared to wild-type V2, whereas the level of aggregates came to a similar after treatment with MG132, which indicates that the S71A mutation might affect 26S proteasome-mediated degradation of V2 aggregates. Meanwhile, heterologous expression of V2S71A from a Potato virus X vector induced mild symptoms compared to wild-type V2, delay of virus infection associated with mild symptoms was observed in plants inoculated with TYLCV-S71A, which indicates that the amino acid on position 71 is also involved in the pathogenicity of V2. To the best of our knowledge, this report is the fir...Continue Reading

References

Jan 4, 2001·Virus Research·E Moriones, J Navas-Castillo
Dec 31, 2003·Cell·Jeffrey M VargasonTraci M Tanka Hall
Apr 7, 2007·The Journal of General Virology·Simon E BullJohn Stanley
Jan 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Efrat GlickYedidya Gafni
Jun 19, 2008·The Journal of General Virology·Chaozheng ZhangBingsheng Qiu
Apr 28, 2009·Journal of Neural Transmission·A NakashimaA Ota
Feb 4, 2010·Molecular Plant-microbe Interactions : MPMI·Inmaculada GonzálezTomás Canto
Jan 1, 2003·Molecular Plant Pathology·Yedidya Gafni
Jul 14, 2010·Molecular Plant Pathology·Juan Antonio Díaz-PendónJesús Navas-Castillo
Oct 25, 2011·Molecular Plant Pathology·Karen-Beth G ScholthofGary D Foster
Apr 26, 2013·Molecular Plant Pathology·Vincent N Fondong
Oct 9, 2013·Nature Reviews. Microbiology·Linda Hanley-BowdoinShahid Mansoor

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Datasets Mentioned

BETA
GU111505

Methods Mentioned

BETA
two-hybrid
Co-Immunoprecipitation
bimolecular fluorescence complementation
PCR
Co-IP
confocal microscopy
Fluorescence

Software Mentioned

BiFC

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