Single-amino-acid mutation in the HA alters the recognition of H9N2 influenza virus by a monoclonal antibody

Biochemical and Biophysical Research Communications
Jihui PingH Chen

Abstract

We explored the molecular basis of antigenic variation by comparing two H9N2 subtype avian influenza viruses, A/Chicken/Shandong/6/96 (CK/SD/6) and A/Chicken/Guangxi/10/99 (CK/GX/10), that react differently to a monoclonal antibody C/B3. To assess the genetic basis for this antigenic difference, we used reverse genetics to generate a series of chimera and mutants of these two viruses. We found that a single-amino-acid substitution of asparagine for serine at position 145 (S145N) in the HA protein prevents the reaction of CK/SD/6 virus with C/B3. Substitution of serine for asparagine at the same position (N145S) enables the CK/GX/10 to react with C/B3 in hemaglutinin inhibition, immunofluorescence and neutralization assays. We further demonstrated that the amino acid N145 in the H9 HA protein is glycosylated. Our results provide experimental evidence that the glycosylation of HA oligosaccharide attachment sites implicated in antibody binding could have a role in antigenic variation.

References

Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J J SkehelD C Wiley
Aug 25, 2001·Rinsho byori. The Japanese journal of clinical pathology·A Oya
Sep 20, 2001·The Journal of General Virology·E TsuchiyaK Nakamura
Jul 3, 2002·The Journal of Clinical Investigation·Peter Palese, Adolfo García-Sastre

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Citations

Mar 5, 2016·Archives of Virology·Feifei GeJinping Zhou
Mar 31, 2009·Biochemical and Biophysical Research Communications·Sheng-Fan WangYi-Ming Arthur Chen
Jun 25, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Duong Mai ThuyJuliet E Bryant
Nov 8, 2018·Emerging Microbes & Infections·Thomas P PeacockMunir Iqbal
Dec 25, 2019·Cold Spring Harbor Perspectives in Medicine·Silvia Carnaccini, Daniel R Perez
Aug 24, 2021·The Journal of General Virology·Phoebe Stevenson-LeggettErica Bickerton

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