Single Amino Acid Mutations Affect Zika Virus Replication In Vitro and Virulence In Vivo.

Viruses
Nicole M ColletteMonica Borucki

Abstract

The 2014-2016 Zika virus (ZIKV) epidemic in the Americas resulted in large deposits of next-generation sequencing data from clinical samples. This resource was mined to identify emerging mutations and trends in mutations as the outbreak progressed over time. Information on transmission dynamics, prevalence, and persistence of intra-host mutants, and the position of a mutation on a protein were then used to prioritize 544 reported mutations based on their ability to impact ZIKV phenotype. Using this criteria, six mutants (representing naturally occurring mutations) were generated as synthetic infectious clones using a 2015 Puerto Rican epidemic strain PRVABC59 as the parental backbone. The phenotypes of these naturally occurring variants were examined using both cell culture and murine model systems. Mutants had distinct phenotypes, including changes in replication rate, embryo death, and decreased head size. In particular, a NS2B mutant previously detected during in vivo studies in rhesus macaques was found to cause lethal infections in adult mice, abortions in pregnant females, and increased viral genome copies in both brain tissue and blood of female mice. Additionally, mutants with changes in the region of NS3 that interface...Continue Reading

References

Apr 15, 2003·The Journal of General Virology·Peter J BredenbeekWilly J M Spaan
Sep 7, 2007·Journal of Immunological Methods·T Kamala
Aug 6, 2008·Emerging Infectious Diseases·Robert S LanciottiMark R Duffy
Nov 1, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Justin R PriggeNicole Meissner
Mar 26, 2016·Science·Nuno Rodrigues FariaPedro F C Vasconcelos
Apr 14, 2016·Cell Host & Microbe·Helen M LazearMichael S Diamond
Apr 19, 2016·Emerging Infectious Diseases·Robert S LanciottiLeticia Del Carmen Castillo Signor
Apr 19, 2016·Nature Structural & Molecular Biology·Hao SongGeorge F Gao
Apr 20, 2016·Cell Host & Microbe·Lulan WangGenhong Cheng
Apr 20, 2016·PLoS Neglected Tropical Diseases·Matthew T AliotaJorge E Osorio
Apr 26, 2016·PLoS Neglected Tropical Diseases·John LednickyJ Glenn Morris
May 7, 2016·PLoS Neglected Tropical Diseases·Stuart D DowallRoger Hewson
May 14, 2016·Cell Host & Microbe·Xuping XiePei-Yong Shi
May 24, 2016·Cell Host & Microbe·Alesha GrantAdolfo García-Sastre
Sep 3, 2016·PLoS Neglected Tropical Diseases·Manuela SironiRachele Cagliani
Sep 30, 2016·The New England Journal of Medicine·Sankar SwaminathanMarc R Couturier
Dec 23, 2016·PLoS Neglected Tropical Diseases·Marina Galvão BuenoMarcia Chame
Jan 10, 2017·Nature Structural & Molecular Biology·Vidya Mangala PrasadMichael G Rossmann
Mar 7, 2017·Science Advances·Ryuta UrakiErol Fikrig
Mar 21, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Arunachalam RamaiahVaithilingaraja Arumugaswami
Apr 27, 2017·Journal of Virological Methods·Sharton Vinicius Antunes CoelhoLuciana Barros de Arruda
May 26, 2017·Nature·Hayden C MetskyPardis C Sabeti
Jul 21, 2017·Emerging Infectious Diseases·Elisabeth R Krow-LucalAllyn K Nakashima
Jan 7, 2018·Science Immunology·Laura J YockeyAkiko Iwasaki
Jan 31, 2018·Nature Communications·Hongjie XiaPei-Yong Shi
Dec 19, 2018·Nature Communications·Konstantin A TsetsarkinAlexander G Pletnev
Apr 18, 2019·PLoS Neglected Tropical Diseases·Anna S JaegerMatthew T Aliota
Jul 31, 2019·Nucleic Acids Research·Shan XuLei Shi
Aug 5, 2020·Proceedings of the National Academy of Sciences of the United States of America·Chao ShanPei-Yong Shi

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Citations

Jul 3, 2021·Tropical Medicine and Infectious Disease·Youssef A Kousa, Reafa A Hossain

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Datasets Mentioned

BETA
KU501215
KX087101
KX377336.1

Methods Mentioned

BETA
PCR
flow cytometry
FACS
flow-cytometry
dissection

Software Mentioned

GraphPad Prism
FlowJo

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