Single and combined effects of carbamazepine and vinpocetine on depolarization-induced changes in Na+, Ca2+ and glutamate release in hippocampal isolated nerve endings

Neurochemistry International
María SitgesVladimir Nekrassov

Abstract

The single and combined effects of carbamazepine and vinpocetine on the release of the excitatory amino acid neurotransmitter glutamate, on the rise in internal Na+ (Na(i), as determined with SBFI), and on the rise in internal Ca2+ (Ca(i), as determined with fura-2) induced by an increased permeability of presynaptic Na+ channels, with veratridine, or by an increased permeability of presynaptic Ca2+ channels with high K+, were investigated in isolated hippocampal nerve endings. The present study shows that carbamazepine and vinpocetine, both inhibit dose dependently the release of preloaded [3H]Glu induced by veratridine. However, carbamazepine is two orders of magnitude less potent than vinpocetine. The calculated IC(50)'s for carbamazepine and vinpocetine to inhibit veratridine-induced [3H]Glu release are 200 and 2 microM, respectively. Consistently 150 microM carbamazepine and 1.5 microM vinpocetine reduce the veratridine-induced rise in Na(i) in a similar extent. The single effects of carbamazepine and of vinpocetine on the presynaptic Na+ channel mediated responses, namely the rise in Na(i) and the release of Glu induced by veratridine, are additive. Responses that depend on the entrance of external Ca2+ via presynaptic Ca...Continue Reading

References

Jun 1, 1991·Developmental Medicine and Child Neurology·I ForsytheR McGuire
Jan 1, 1987·Pharmacology, Biochemistry, and Behavior·V J DeNoble
Aug 1, 1987·Archives of Neurology·M O'DoughertyP Walson
Oct 1, 1987·International Clinical Psychopharmacology·J Z Bhatti, I Hindmarch
Jan 1, 1985·European Journal of Clinical Pharmacology·Z Subhan, I Hindmarch
Apr 1, 1995·Journal of Neuroscience Research·M Sitges, A Reyes
May 1, 1993·Journal of Neurophysiology·R J SayerW E Crill
Aug 1, 1996·Neurochemical Research·R Rodríguez, M Sitges
May 26, 1998·Brain Research. Brain Research Reviews·D S Ragsdale, M Avoli
Dec 11, 1999·Journal of Child Neurology·W T Seidel, W G Mitchell
Jan 12, 2002·British Journal of Pharmacology·A RobichaudC C Chan
Mar 1, 2003·Epilepsy & Behavior : E&B·K J Meador
Oct 19, 2004·Lancet Neurology·Christian E ElgerMartin Kurthen
Nov 18, 2004·Clinical Neurophysiology : Official Journal of the International Federation of Clinical Neurophysiology·Maria Sitges, V Nekrassov

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Citations

May 24, 2007·Transplantation·Mark-Hugo J MaathuisRutger J Ploeg
Sep 17, 2008·Neurochemistry International·K TárnokU L M Eisel
Feb 27, 2007·Toxicology and Applied Pharmacology·Zhi-Fang ChaiYong-Hua Ji
Feb 1, 2012·Journal of Neurochemistry·Blanca I Aldana, María Sitges
Jul 28, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Maria B MorettoJoao B T Rocha

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