Single-biomolecule kinetics: the art of studying a single enzyme

Annual Review of Analytical Chemistry
Victor I ClaessenAlan E Rowan

Abstract

The potential of single-enzyme studies to unravel the complex energy landscape of these polymeric catalysts is the next critical step in enzymology. From its inception in Rotman's emulsion experiments in the 1960s, the field of single-molecule enzymology has now advanced into the time-resolved age. Technological advances have enabled individual enzymatic turnover reactions to be observed with a millisecond time resolution. A number of initial studies have revealed the underlying static and dynamic disorder in the catalytic rates originating from conformational fluctuations. Although these experiments are still in their infancy, they may be able to relate the topography of the energy landscape to the biological function and regulation of enzymes. This review summarizes some of the experimental techniques and data-analysis methods that have been used to study individual enzyme molecules in search of a deeper understanding of their kinetics.

References

Apr 6, 1990·Science·W DenkW W Webb
Mar 15, 1995·Experientia·H Frauenfelder
Dec 4, 1998·Science·H P LuX S Xie
Jul 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·L Edman, R Rigler
Feb 23, 2002·Science·Elan Zohar EisenmesserDorothee Kern
Jul 25, 2003·Trends in Biochemical Sciences·Leo C James, Dan S Tawfik
Dec 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·B ROTMAN
Jan 20, 2004·Nature Structural & Molecular Biology·Takayuki NishizakaKazuhiko Kinosita
Jan 30, 2004·Nature·Hiroyasu ItohKazuhiko Kinosita
Sep 24, 2004·Proteins·K GunasekaranRuth Nussinov
Sep 28, 2004·The Journal of Chemical Physics·James B Witkoskie, Jianshu Cao
Dec 25, 2004·Angewandte Chemie·Kelly VeloniaFrans C de Schryver
Feb 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ophir FlomenbomJoseph Klafter
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·H Jane Dyson, Peter E Wright
Mar 15, 2005·Biophysical Journal·Ophir FlomenbomAttila Szabo
Mar 15, 2005·Biophysical Journal·Alan I Lee, James P Brody
Jul 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hans-Philipp LerchAlexander S Mikhailov
Jan 13, 2006·Nano Letters·Joshua B BallardMartin Gruebele
Jan 18, 2006·Nature Chemical Biology·Brian P EnglishX Sunney Xie
Feb 24, 2006·Chemical Communications : Chem Comm·Hans EngelkampAlan E Rowan
Mar 21, 2006·Optics Letters·Ted A LaurenceThomas Huser
Jun 13, 2006·Chemical Communications : Chem Comm·Nikos S HatzakisRoeland J M Nolte
Jul 21, 2006·The Journal of Physical Chemistry. B·Lucas P Watkins, Haw Yang
Aug 10, 2006·Chemical Reviews·David D BoehrPeter E Wright
Aug 10, 2006·Chemical Reviews·R Derike Smiley, Gordon G Hammes
Aug 10, 2006·Nature Methods·Michael J RustXiaowei Zhuang
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Sep 16, 2006·Science·Michele Vendruscolo, Christopher M Dobson
Sep 19, 2006·Biophysical Journal·Samuel T HessMichael D Mason
May 4, 2007·Chemical Communications : Chem Comm·Bernard T KellyAndrew D Griffiths
Jul 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Eric D WattJ Patrick Loria
Jul 21, 2007·Angewandte Chemie·J FöllingS W Hell
Oct 25, 2007·Journal of the American Chemical Society·Cristina FlorsJohan Hofkens
Oct 30, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hans H GorrisDavid R Walt
Nov 23, 2007·Journal of the American Chemical Society·Gert De CremerDirk E De Vos
Dec 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Akinori Baba, Tamiki Komatsuzaki
Dec 14, 2007·Nature·Katherine Henzler-Wildman, Dorothee Kern
Jan 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Chun-Biu LiTamiki Komatsuzaki
Feb 28, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sofya KuznetsovaGerard W Canters

❮ Previous
Next ❯

Citations

May 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Hubert M PiwonskiGilad Haran
Jul 16, 2013·FEBS Letters·Anne SchwabeFrank J Bruggeman
Nov 28, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Krutika Bavishi, Nikos S Hatzakis
Sep 16, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Raphaela B Liebherr, Hans H Gorris
Oct 16, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Klaus MathwigLiza Rassaei
Jun 22, 2011·Biochimie·David FangeMåns Ehrenberg
Sep 28, 2012·Science China. Life Sciences·ChuanPeng LiuQiMing Zhou
Jan 15, 2014·Biophysical Journal·Haifeng Yuan, Michel Orrit
Feb 24, 2018·Nature Communications·Tal RobinMichael Urbakh
Jan 24, 2014·Advanced Materials·Kannan Balasubramanian, Klaus Kern
Oct 3, 2013·Chemical Society Reviews·Kris P F JanssenJohan Hofkens
Mar 13, 2014·Proceedings of the National Academy of Sciences of the United States of America·Shlomi ReuveniJoseph Klafter
Oct 11, 2014·Chemical Communications : Chem Comm·Namik AkkilicNusrat J M Sanghamitra
Jan 10, 2014·Chemical Communications : Chem Comm·S SerdjukowT Carell
Jan 31, 2013·Chembiochem : a European Journal of Chemical Biology·Pietro Gatti-Lafranconi, Florian Hollfelder
Jun 25, 2021·Chemical Science·Jack W JordanAndrei N Khlobystov
Dec 3, 2011·ACS Nano·Tatyana G TerentyevaKerstin Blank
Jan 15, 2013·The Journal of Physical Chemistry. B·Tatyana G TerentyevaChun-Biu Li
Jan 22, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Johannes A MengesGregor Jung
Dec 20, 2018·The Journal of Physical Chemistry Letters·Yanjing GaoDi Li
Jan 17, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Steven E F KleijnMarc T M Koper
Jun 1, 2013·Analytical Chemistry·Aoshuang XuEdward S Yeung
Jul 16, 2014·The Journal of Physical Chemistry. B·Desheng Zheng, H Peter Lu
Nov 10, 2018·The Journal of Physical Chemistry Letters·Yan XuDi Li

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Methods Mentioned

BETA
nuclear magnetic resonance
fluorescence
confocal microscopy
fluorescence correlation spectroscopy
FCS
X-ray
Assay
electrophoresis
flow
imaging techniques

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