Single-carbon discrimination by selected peptides for individual detection of volatile organic compounds

Scientific Reports
Soomi JuHyunjung Yi

Abstract

Although volatile organic compounds (VOCs) are becoming increasingly recognized as harmful agents and potential biomarkers, selective detection of the organic targets remains a tremendous challenge. Among the materials being investigated for target recognition, peptides are attractive candidates because of their chemical robustness, divergence, and their homology to natural olfactory receptors. Using a combinatorial peptide library and either a graphitic surface or phenyl-terminated self-assembled monolayer as relevant target surfaces, we successfully selected three interesting peptides that differentiate a single carbon deviation among benzene and its analogues. The heterogeneity of the designed target surfaces provided peptides with varying affinity toward targeted molecules and generated a set of selective peptides that complemented each other. Microcantilever sensors conjugated with each peptide quantitated benzene, toluene and xylene to sub-ppm levels in real time. The selection of specific receptors for a group of volatile molecules will provide a strong foundation for general approach to individually monitoring VOCs.

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Citations

Jul 23, 2015·ACS Applied Materials & Interfaces·Jae-Hun Kim, Sang Sub Kim
Jul 28, 2017·Advanced Functional Materials·Abid HussainAna C A Roque
Nov 8, 2017·Macromolecular Rapid Communications·Niels Ten BrummelhuisHans G Börner
Aug 29, 2017·ACS Combinatorial Science·Mina OkochiKiyoshi Toko

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Methods Mentioned

BETA
peptide library
phage-display
chip

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