Single carbon nanotube-based reversible regulation of biological motor activity

ACS Nano
Yuichi InoueAkihiko Ishijima

Abstract

Because of their small size and high thermal conductivity, carbon nanotubes (CNTs) are excellent candidates for exploring heat transfer at the level of individual molecules in biological research. With a view toward examining the thermal regulation of single biomolecules, we here developed single CNTs as a new platform for observing the motile activity of myosin motors. On multiwall CNTs (diameter ∼170 nm; length ∼10 μm) coated with skeletal-muscle myosin, the ATP-driven sliding of single actin filaments was clearly observable. The normal sliding speed was ∼6 μm/s. Locally irradiating one end of the CNT with a red laser (642 nm), without directly irradiating the active myosin motors, accelerated the sliding speed to ∼12 μm/s, indicating the reversible activation of protein function on a single CNT in real time. The temperature along the CNT, which was estimated from the temperature-dependence of the sliding speed, decreased with the distance from the irradiated spot. Using these results with the finite element method, we calculated a first estimation of the thermal conductivity of multiwall CNTs in solution, as 1540 ± 260 (Wm(-1) K(-1)), which is consistent with the value estimated from the width dependency of multiwall CNTs an...Continue Reading

References

Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Y Y ToyoshimaJ A Spudich
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S J Kron, J A Spudich
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·H KatoS Ishiwata
Jun 3, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A F Huxley
Sep 16, 2000·Physical Review Letters·S BerberD Tomanek
Apr 3, 2001·Nature Biotechnology·R Weissleder
Apr 20, 2001·Cell Motility and the Cytoskeleton·K Kawaguchi, S Ishiwata
Dec 12, 2001·Physical Review Letters·P KimP L McEuen
Aug 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·Nadine Wong Shi KamHongjie Dai
Aug 11, 2005·Physical Review Letters·Motoo FujiiTetsuo Shimizu
Feb 28, 2007·Biophysical Journal·Steven M Block
Jul 21, 2007·Science·Martin G L van den Heuvel, Cees Dekker
Dec 22, 2009·Angewandte Chemie·Youichi TsuchiyaSeiji Shinkai
Feb 1, 2011·European Biophysics Journal : EBJ·Matthew A B BakerRichard M Berry
Oct 23, 2012·Journal of the American Chemical Society·Tatsuya MurakamiHiroshi Imahori
Apr 9, 2013·ACS Nano·Chaenyung ChaAli Khademhosseini
May 2, 2013·Journal of the American Chemical Society·Patrick C SimsGregory A Weiss
May 2, 2013·Journal of the American Chemical Society·Tivoli J OlsenGregory A Weiss
Jan 3, 2014·Nano Letters·Aurélien SikoraWinfried Teizer

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Citations

Feb 12, 2019·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Rachel Andorfer, Joshua D Alper
Mar 1, 2016·Biophysics Reviews·Yuichi Inoue, Akihiko Ishijima

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