Single-cell analysis defines the divergence between the innate lymphoid cell lineage and lymphoid tissue-inducer cell lineage

Nature Immunology
Isabel E IshizukaRachel Golub

Abstract

The precise lineage relationship between innate lymphoid cells (ILCs) and lymphoid tissue-inducer (LTi) cells is poorly understood. Using single-cell multiplex transcriptional analysis of 100 lymphoid genes and single-cell cultures of fetal liver precursor cells, we identified the common proximal precursor to these lineages and found that its bifurcation was marked by differential induction of the transcription factors PLZF and TCF1. Acquisition of individual effector programs specific to the ILC subsets ILC1, ILC2 and ILC3 was initiated later, at the common ILC precursor stage, by transient expression of mixed ILC1, ILC2 and ILC3 transcriptional patterns, whereas, in contrast, the development of LTi cells did not go through multilineage priming. Our findings provide insight into the divergent mechanisms of the differentiation of the ILC lineage and LTi cell lineage and establish a high-resolution 'blueprint' of their development.

References

Jul 12, 2002·Developmental Cell·Toshihiro MiyamotoKoichi Akashi
Mar 3, 2010·Annual Review of Immunology·Jinfang ZhuWilliam E Paul
Mar 5, 2010·Nature·Daniel R NeillAndrew N J McKenzie
Dec 24, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Masashi TachibanaIchiro Taniuchi
Jan 24, 2012·Nature Immunology·See Heng WongAndrew N J McKenzie
Mar 21, 2012·The Journal of Experimental Medicine·Marie CherrierGérard Eberl
Jan 22, 2013·Nature·Christoph S N KloseAndreas Diefenbach
Apr 9, 2013·Immunity·Nidhi MalhotraUNKNOWN Immunological Genome Project Consortium
Apr 23, 2013·Immunity·Qi YangAvinash Bhandoola
Sep 17, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lisa A MielkeGabrielle T Belz
Oct 22, 2013·Nature Immunology·Chauncey J SpoonerHarinder Singh
Jan 15, 2014·The Journal of Experimental Medicine·Nicolas SerafiniJames P Di Santo
Feb 11, 2014·Nature·Michael G ConstantinidesAlbert Bendelac
Mar 29, 2014·Nature·Serge A van de PavertHenrique Veiga-Fernandes
Aug 6, 2014·The Journal of Experimental Medicine·Cyril SeilletSebastian Carotta
Sep 23, 2014·Immunity·Andreas DiefenbachShigeo Koyasu
Dec 17, 2014·Nature Immunology·Jennifer K BandoRichard M Locksley
Dec 17, 2014·Seminars in Immunopathology·Kazuyo Moro, Shigeo Koyasu
Mar 25, 2015·Cell Reports·Wei XuHenrique Veiga-Fernandes
Apr 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Michael G ConstantinidesAlbert Bendelac
May 13, 2015·The Journal of Experimental Medicine·Jennifer A WalkerAndrew N J McKenzie

❮ Previous
Next ❯

Citations

Apr 6, 2016·Frontiers in Immunology·Anja Fuchs
May 12, 2016·Annual Review of Immunology·Isabel E IshizukaAlbert Bendelac
Sep 1, 2016·Frontiers in Immunology·Katrijn NeytBart N Lambrecht
Sep 23, 2016·Frontiers in Immunology·Manuela Buettner, Matthias Lochner
Feb 6, 2017·Nature Reviews. Rheumatology·Medya M ShikhagaieHergen Spits
Feb 19, 2016·European Journal of Immunology·Hye Young KimRosemarie H Dekruyff
Dec 10, 2016·Immunology·Felipe Melo-Gonzalez, Matthew R Hepworth
Jan 18, 2017·Trends in Immunology·Karlynn E NeuAly A Khan
Mar 28, 2017·Experimental Cell Research·Francisco Verdeguer, Myriam Aouadi
Jan 22, 2017·Protein & Cell·Chao Zhong, Jinfang Zhu
Jun 29, 2017·Immunological Reviews·Hadi Maazi, Omid Akbari
Jun 11, 2017·FEBS Letters·Ilaria LungerMichael A Rieger
Mar 8, 2018·The Journal of Experimental Medicine·Na LiFrits Koning
Jun 22, 2016·Nature Immunology·Erin C Zook, Barbara L Kee
Jun 22, 2016·Nature Immunology·Christoph S N Klose, David Artis
Jun 22, 2016·Nature Immunology·Eric VivierGabrielle T Belz
Oct 7, 2017·Current Allergy and Asthma Reports·Paul J MaglioneCharlotte Cunningham-Rundles
Mar 5, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hong-Cheng WangXiao-Hong Sun
Dec 1, 2017·The Journal of Experimental Medicine·Christelle HarlyAvinash Bhandoola
Dec 19, 2017·Frontiers in Immunology·Giuseppe SciumèJohn J O'Shea
Dec 5, 2017·Frontiers in Immunology·Qiutong HuangGabrielle T Belz
Dec 19, 2017·Frontiers in Immunology·Bobby W S LiRudi W Hendriks
Oct 31, 2018·Nature Immunology·Hiroyuki HosokawaEllen V Rothenberg
Dec 7, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yotam E Bar-EphraimReina E Mebius
Feb 21, 2019·International Immunology·Yohei OkuboShinsuke Taki
Apr 27, 2019·Annual Review of Immunology·Ana CumanoPablo Pereira
Dec 10, 2019·The Journal of Experimental Medicine·Maryam GhaediFumio Takei
Nov 5, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bernard C LoKelly M McNagny
Jun 3, 2016·International Journal of Hematology·Takahiro Maeda
Nov 5, 2016·European Journal of Immunology·Robrecht CannoodtYvan Saeys
Nov 5, 2016·The Journal of Experimental Medicine·Elia D Tait Wojno, David Artis
May 8, 2018·The Journal of Experimental Medicine·Satoshi KogaKazuyo Moro
Jan 11, 2018·Scientific Reports·Sara M ParigiEduardo J Villablanca
Jun 28, 2019·Frontiers in Immunology·Liang ShaoAlbert K W Lie
Aug 1, 2020·Frontiers in Immunology·Vladislava Stokic-TrticaChristoph S N Klose
Jun 27, 2019·Immunotherapy·Shunfeng Hu, Xin Wang
Jun 7, 2017·Proceedings of the National Academy of Sciences of the United States of America·William J R LongabaughEllen V Rothenberg
May 24, 2017·Immunogenetics·Madeleine SuffiottiDavid Gfeller
Nov 1, 2017·Frontiers in Immunology·David R Withers, Matthew R Hepworth
Oct 9, 2018·Immunological Reviews·Antonia WallrappVijay K Kuchroo
Jul 12, 2020·Mucosal Immunology·Marie CherrierRachel Golub

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
FACS
FCS
PCR
Assay
chip

Software Mentioned

custom
GraphPad
FlowJo
Prism
NMF
Biomark
R

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.