Single-cell analysis of gene expression in the nervous system. Measurements at the edge of chaos

Molecular Neurobiology
D K O'Dowd, M A Smith

Abstract

The characteristic functions of tissues and organs result from the integrated activity of individual cells. Nowhere is this more evident than in the nervous system, where the activities of single neurons communicating via electrical and chemical signals mediate complex functions, such as learning and memory. The past decade has seen an explosion in the identification of genes encoding proteins, such as voltage-gated channels and neurotransmitter receptors, responsible for neuronal excitability. These studies have highlighted the fact that even within a neuroanatomically defined region, the coexistence of multiple cell types makes it difficult, if not impossible, to correlate patterns of gene expression with function. The recent development of techniques sensitive enough to study gene expression at the single-cell level promises to break this bottleneck to our further understanding. Using examples taken from our own laboratories and the work of others, we review these techniques, their application, and discuss some of the difficulties associated with the interpretation of the data.

References

Aug 1, 1992·Neuron·B LambolezJ Rossier
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D J SurmeierS T Kitai
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J EberwineP Coleman
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R N Van GelderJ H Eberwine
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G GillilandH F Bunn
Nov 11, 1990·Nucleic Acids Research·W RychlikR E Rhoads
Jun 1, 1995·Neuron·N J Sucher, D L Deitcher
Jan 1, 1995·Annual Review of Neuroscience·M A Bowe, J R Fallon
Jun 1, 1995·Current Opinion in Cell Biology·R D Kornberg, Y Lorch
Mar 1, 1995·Neurochemistry International·F F JohansenJ Rossier
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·K MiyashiroJ Eberwine
Mar 1, 1994·Visual Neuroscience·E V Grigorenko, H H Yeh
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·B LambolezS Hestrin

❮ Previous
Next ❯

Citations

Jan 25, 2012·Cellular and Molecular Neurobiology·Ruby KwongMichal Janitz
Mar 29, 2001·Autonomic Neuroscience : Basic & Clinical·J K Phillips, J Lipski
Apr 24, 2012·Journal of Environmental and Public Health·Paula Valencia Moulton, Wei Yang
Jan 9, 1999·Medical Hypotheses·E R Bolte
Jan 18, 2015·Journal of Pharmaceutical and Biomedical Analysis·Nurullah ŞanlıCraig Lunte
Sep 2, 1999·Biochemical and Biophysical Research Communications·B ChetsawangP Govitrapong
Aug 2, 2008·Journal of Neuroscience Methods·Tereance A MyersMario T Philipp
Mar 13, 2019·The Nigerian Postgraduate Medical Journal·Ochuwa Adiketu BabahBosede B Afolabi
Sep 19, 2000·American Journal of Botany·W T Pockman, J S Sperry
Mar 28, 2012·The Plant Journal : for Cell and Molecular Biology·Akira Oikawa, Kazuki Saito
Feb 5, 2016·Journal of Neurophysiology·Soleil S SchutteDiane K O'Dowd
Jun 5, 1998·The American Journal of Physiology·S HuberM Horster
Jul 24, 2001·Amyotrophic Lateral Sclerosis and Other Motor Neuron Disorders : Official Publication of the World Federation of Neurology, Research Group on Motor Neuron Diseases·V SilaniG Scarlato
Oct 20, 2007·The Journal of Physiology·Ted D HoydaAlastair V Ferguson
May 4, 2010·Trends in Biotechnology·Daojing Wang, Steven Bodovitz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.