Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development.

Proceedings of the National Academy of Sciences of the United States of America
Marc A GavinAlexander Y Rudensky

Abstract

Forkhead winged-helix transcription factor Foxp3 serves as the dedicated mediator of the genetic program governing CD25+CD4+ regulatory T cell (T(R)) development and function in mice. In humans, its role in mediating T(R) development has been controversial. Furthermore, the fate of T(R) precursors in FOXP3 deficiency has yet to be described. Making use of flow cytometric detection of human FOXP3, we have addressed the relationship between FOXP3 expression and human T(R) development. Unlike murine Foxp3- T cells, a small subset of human CD4+ and CD8+ T cells transiently up-regulated FOXP3 upon in vitro stimulation. Induced FOXP3, however, did not alter cell-surface phenotype or suppress T helper 1 cytokine expression. Furthermore, only ex vivo FOXP3+ T(R) cells persisted after prolonged culture, suggesting that induced FOXP3 did not activate a T(r) developmental program in a significant number of cells. FOXP3 flow cytometry was also used to further characterize several patients exhibiting symptoms of immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) with or without FOXP3 mutations. Most patients lacked FOXP3-expressing cells, further solidifying the association between FOXP3 deficiency and immune dy...Continue Reading

References

Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M F LyonE Wright
Dec 20, 2000·The Journal of Clinical Investigation·T A ChatilaA M Bowcock
Apr 12, 2001·European Journal of Immunology·L A StephensF Powrie
Aug 2, 2001·Human Genetics·J T den Dunnen, S E Antonarakis
Jan 11, 2003·Science·Shohei HoriShimon Sakaguchi
Mar 4, 2003·Nature Immunology·Jason D FontenotAlexander Y Rudensky
Mar 4, 2003·Nature Immunology·Roli KhattriFred Ramsdell
Nov 5, 2003·The Journal of Clinical Investigation·Mindi R WalkerSteven F Ziegler
Mar 24, 2004·Seminars in Immunology·Clare Baecher-AllanDavid A Hafler
Jul 15, 2004·PLoS Biology·Kyra Oswald-RichterDerya Unutmaz
Aug 17, 2004·Immunity·Chyi-Song HsiehAlexander Y Rudensky
Sep 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·E Ashley MosemanWei Chen
Oct 7, 2004·International Immunology·Haruhiko YagiShimon Sakaguchi
Dec 29, 2004·Human Immunology·Mary E MorganRené E M Toes
Mar 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mindi R WalkerJane H Buckner
Mar 23, 2005·Immunity·Jason D FontenotAlexander Y Rudensky
May 5, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Clare Baecher-AllanDavid A Hafler
May 10, 2005·Transplantation·Reginald M GorczynskiIvo Boudakov
May 20, 2005·European Journal of Immunology·Giovanna RoncadorAlison H Banham
Oct 8, 2005·The Journal of Clinical Investigation·Sarah E AllanMegan K Levings
Oct 26, 2005·Nature Immunology·Karsten KretschmerHarald von Boehmer
Mar 15, 2006·Nature Immunology·Chyi-Song HsiehAlexander Y Rudensky

❮ Previous
Next ❯

Citations

Mar 29, 2013·Archivum Immunologiae Et Therapiae Experimentalis·Maciej Kaczorowski, Marek Jutel
Oct 27, 2009·Cancer Immunology, Immunotherapy : CII·Andrew J RechRobert H Vonderheide
Jun 4, 2011·Cancer Immunology, Immunotherapy : CII·David M FoureauJonathan C Salo
Aug 12, 2006·Springer Seminars in Immunopathology·Troy R Torgerson
Sep 11, 2010·Cancer Metastasis Reviews·Maria Angelica Ehara WatanabeJulio Cesar Voltarelli
Feb 14, 2012·Targeted Oncology·Christine Ménétrier-CauxWeiping Zou
Feb 3, 2009·Immunologic Research·Dorothy K SojkaDeborah J Fowell
Jan 25, 2013·Immunologic Research·Chrysoulla PieridesErnesto Oviedo-Orta
Sep 11, 2009·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Yasunori ItoToshio Miyawaki
May 28, 2008·Immunology and Cell Biology·Heli TuovinenT Petteri Arstila
Feb 9, 2011·Immunology and Cell Biology·Makoto Miyara, Shimon Sakaguchi
Oct 31, 2007·Nature Clinical Practice. Rheumatology·Xavier Valencia, Peter E Lipsky
May 21, 2013·Nature Immunology·Esther Bandala-SanchezLeonard C Harrison
Feb 3, 2007·Nature Immunology·Wen LinTalal A Chatila
Apr 19, 2007·Nature Immunology·Ye Zheng, Alexander Y Rudensky
Mar 8, 2007·Nature Medicine·Tyler J Curiel
Jan 1, 2013·Nature Reviews. Drug Discovery·Harald von Boehmer, Carolin Daniel
Feb 24, 2007·Nature Reviews. Immunology·Arne N AkbarDerek C Macallan
Mar 24, 2007·Nature Reviews. Immunology·Daniel J Campbell, Steven F Ziegler
Jun 21, 2008·Nature Reviews. Immunology·Dario A A VignaliCreg J Workman
Sep 3, 2010·Nature Reviews. Rheumatology·Clare A Notley, Michael R Ehrenstein
Jun 27, 2007·Immunology and Cell Biology·Christian LangeFelix Bischof
Nov 7, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sarah E AllanMegan K Levings
Sep 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Chrysoula I LiakouPadmanee Sharma
Nov 28, 2008·Proceedings of the National Academy of Sciences of the United States of America·Fabian Flores-BorjaMichael R Ehrenstein
Jan 4, 2007·The Journal of Experimental Medicine·Suchita NadkarniMichael R Ehrenstein
Jul 16, 2008·AIDS Research and Human Retroviruses·Aki HojiOtto O Yang
Jul 18, 2012·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Li-Na JiangZhi-Yong Sheng
Mar 13, 2009·Human Reproduction Update·Leigh R GuerinSarah A Robertson
Jul 25, 2009·International Immunology·Grégory NoëlDenis Bruniquel
Aug 4, 2009·Journal of Molecular Cell Biology·Wanjun Chen, Joanne E Konkel
Sep 5, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Julien ZuberLucienne Chatenoud
Dec 25, 2012·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Julien ZuberYvon Lebranchu
Oct 12, 2011·Current Opinion in Allergy and Clinical Immunology·James W Verbsky, Talal A Chatila
Apr 7, 2007·Current Opinion in Rheumatology·James W Verbsky
Apr 7, 2007·Journal of Immunotherapy·Mojgan AhmadzadehSteven A Rosenberg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Autoimmune Polyendocrine Syndromes

This feed focuses on a rare genetic condition called Autoimmune Polyendocrine Syndromes, which are characterized by autoantibodies against multiple endocrine organs. This can lead to Type I Diabetes.

Autoimmune Polyendocrinopathies

Autoimmune polyendocrinopathies, also called polyglandular autoimmune syndromes (PGASs), or polyendocrine autoimmune syndromes(PASs), are a heterogeneous group of rare diseases characterized by autoimmune activity against more than one endocrine organ, although non-endocrine organs can be affected. Discover the latest research on autoimmune polyendocrinopathies here.