Mar 30, 2016

Single-Cell Analysis of the Dps Response to Oxidative Stress

Journal of Bacteriology
Michela De MartinoAnne S Meyer

Abstract

Microorganisms have developed an elaborate spectrum of mechanisms to respond and adapt to environmental stress conditions. Among these is the expression of dps, coding for the DNA-binding protein from starved cells. Dps becomes the dominant nucleoid-organizing protein in stationary-phase Escherichia coli cells and is required for robust survival under stress conditions, including carbon or nitrogen starvation, oxidative stress, metal exposure, and irradiation. To study the complex regulation of Dps in E. coli, we utilized time-lapse fluorescence microscopy imaging to examine the kinetics, input encoding, and variability of the Dps response in single cells. In the presence of an oxidative stressor, we observed a single pulse of activation of Dps production. Increased concentrations of H2O2 led to increased intensity and duration of the pulse. While lower concentrations of H2O2 robustly activated the Dps response with little effect on the growth rate, higher concentrations of H2O2 resulted in dramatically lower and highly varied growth rates. A comparison of cells exposed to the same concentration of H2O2 revealed that increased levels of Dps expression did not confer a growth advantage, indicating that recovery from stress may r...Continue Reading

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  • Citations5

References

Mentioned in this Paper

Single-Cell Analysis
Microorganism
Metabolic Process, Cellular
Neuro-Oncological Ventral Antigen 2
Gene Expression Regulation, Bacterial
Biological Adaptation to Stress
Biologic Segmentation
Hydrogen Peroxide
Alkalescens-Dispar Group
Enzymes, antithrombotic

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