Single-cell analysis reveals oligoclonality among 'low-count' monoclonal B-cell lymphocytosis.

Leukemia
Mark C LanasaM C Levesque

Abstract

Monoclonal B-cell lymphocytosis (MBL) is a preclinical hematologic syndrome characterized by small accumulations of CD5(+) B lymphocytes. Most MBL share phenotypic characteristics with chronic lymphocytic leukemia (CLL). Although some MBL progress to CLL, most MBL have apparently limited potential for progression to CLL, particularly those MBL with normal absolute B-cell counts ('low-count' MBL). Most CLL are monoclonal and it is not known whether MBL are monoclonal or oligoclonal; this is important because it is unclear whether MBL represent indolent CLL or represent a distinct premalignant precursor before the development of CLL. We used flow cytometry analysis and sorting to determine immunophenotypic characteristics, clonality and molecular features of MBL from familial CLL kindreds. Single-cell analysis indicated four of six low-count MBL consisted of two or more unrelated clones; the other two MBL were monoclonal. 87% of low-count MBL clones had mutated immunoglobulin genes, and no immunoglobulin heavy-chain rearrangements of V(H) family 1 were observed. Some MBL were diversified, clonally related populations with evidence of antigen drive. We conclude that although low-count MBL share many phenotypic characteristics with...Continue Reading

References

Oct 28, 1998·The Journal of Clinical Investigation·F FaisN Chiorazzi
Oct 25, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·I S LossosR Levy
Nov 9, 2000·European Journal of Immunology·K D GeigerR Küppers
Jan 3, 2001·The New England Journal of Medicine·H DöhnerP Lichter
Nov 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·George Adrian CalinCarlo M Croce
Feb 25, 2003·Cytometry. Part B, Clinical Cytometry·Gerald E MartiNeil Caporaso
Dec 21, 2004·Nucleic Acids Research·Marie-Paule LefrancGérard Lefranc
Feb 25, 2005·The New England Journal of Medicine·Nicholas ChiorazziManlio Ferrarini
Jul 27, 2005·British Journal of Haematology·Gerald E MartiUNKNOWN International Familial CLL Consortium
Jun 20, 2007·Leukemia & Lymphoma·Sarah S FungHeather A Leitch
Jul 27, 2007·American Journal of Hematology·J Brice WeinbergMarc C Levesque
Nov 21, 2007·British Journal of Haematology·Gerald MartiRobert F Vogt
Mar 25, 2008·Lancet·G Dighiero, T J Hamblin
Aug 9, 2008·The New England Journal of Medicine·Andy C RawstronPeter Hillmen
Feb 14, 2009·The New England Journal of Medicine·Ola LandgrenNeil E Caporaso

❮ Previous
Next ❯

Citations

Jan 12, 2013·Current Hematologic Malignancy Reports·Andy C Rawstron
Jan 15, 2011·Leukemia·J AlmeidaUNKNOWN Primary Health Care Group of Salamanca for the Study of MBL
Apr 15, 2010·Current Opinion in Hematology·Lynn R GoldinNeil E Caporaso
Aug 8, 2014·International Journal of Hematology·Yoshikane Kikushige, Toshihiro Miyamoto
Dec 17, 2010·Immunologic Research·Mark C Lanasa, J Brice Weinberg
Jul 16, 2010·Leukemia & Lymphoma·Mark C LanasaJ Brice Weinberg
Apr 9, 2013·Hematology/oncology Clinics of North America·Lydia ScarfòPaolo Ghia
Sep 8, 2010·Seminars in Cancer Biology·Lydia ScarfòPaolo Ghia
Sep 21, 2010·Cytometry. Part B, Clinical Cytometry·Wendy G NietoGerald E Marti
Sep 21, 2010·Cytometry. Part B, Clinical Cytometry·Gerald E MartiRobert F Vogt
Sep 21, 2010·Cytometry. Part B, Clinical Cytometry·Erica SalernoElizabeth Raveche
Sep 21, 2010·Cytometry. Part B, Clinical Cytometry·Wendy G NietoUNKNOWN Primary Health Care Group of Salamanca for the Study of MBL
Dec 24, 2014·Genes, Chromosomes & Cancer·Ioannis V KostopoulosStefanos I Papadhimitriou
Oct 9, 2014·BioMed Research International·Christina KalpadakisMaria K Angelopoulou
Mar 17, 2012·Blood·Paolo Ghia, Federico Caligaris-Cappio
Oct 21, 2016·Cell Biology and Toxicology·Michael P ChuLinda M Pilarski
Jul 15, 2011·São Paulo Medical Journal = Revista Paulista De Medicina·Daniel Mazza Matos, Roberto Passetto Falcão
Aug 27, 2010·British Journal of Haematology·Lynn R GoldinNeil E Caporaso
Sep 29, 2019·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Kamila StranskaMichael Doubek
Dec 5, 2019·International Journal of Hematology·Yoshikane Kikushige
Apr 20, 2018·Frontiers in Immunology·Walaa DarwicheHussein Ghamlouch
Dec 7, 2011·Cancer Control : Journal of the Moffitt Cancer Center·Yvonne M Mowery, Mark C Lanasa
Nov 6, 2020·Journal of Clinical and Experimental Hematopathology : JCEH·Yoshikane Kikushige
Nov 9, 2019·Seminars in Oncology·Daphne R FriedmanJ Brice Weinberg

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
flow
PCR
FACS
chromosomal aberrations

Software Mentioned

IMGT
FlowJo

Related Concepts

Related Feeds

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.

Related Papers

The New England Journal of Medicine
Christopher S MulliganStephen P Mulligan
The New England Journal of Medicine
O LandgrenNeil E Caporaso
British Journal of Haematology
Gerald E MartiInternational Familial CLL Consortium
Leukemia & Lymphoma
Mark C LanasaJ Brice Weinberg
© 2022 Meta ULC. All rights reserved