Single-cell analysis reveals transcriptional heterogeneity of neural progenitors in human cortex

Nature Neuroscience
Matthew B JohnsonChristopher A Walsh

Abstract

The human cerebral cortex depends for its normal development and size on a precisely controlled balance between self-renewal and differentiation of diverse neural progenitor cells. Specialized progenitors that are common in humans but virtually absent in rodents, called outer radial glia (ORG), have been suggested to be crucial to the evolutionary expansion of the human cortex. We combined progenitor subtype-specific sorting with transcriptome-wide RNA sequencing to identify genes enriched in human ORG, which included targets of the transcription factor neurogenin and previously uncharacterized, evolutionarily dynamic long noncoding RNAs. Activating the neurogenin pathway in ferret progenitors promoted delamination and outward migration. Finally, single-cell transcriptional profiling in human, ferret and mouse revealed more cells coexpressing proneural neurogenin targets in human than in other species, suggesting greater neuronal lineage commitment and differentiation of self-renewing progenitors. Thus, we find that the abundance of human ORG is paralleled by increased transcriptional heterogeneity of cortical progenitors.

References

Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·A WeigmannW B Huttner
May 29, 2000·Trends in Genetics : TIG·P RiceA Bleasby
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·N UchidaI L Weissman
Nov 4, 2004·Journal of Neuroscience Research·Ayano KawaguchiTakaki Miyata
Apr 13, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zhicheng MoNada Zecevic
Nov 17, 2007·The EMBO Journal·Seongjin SeoKristen L Kroll
Dec 1, 2009·Journal of Neuroscience Methods·Víctor Borrell
Feb 16, 2010·Nature·David V HansenArnold R Kriegstein
Feb 1, 2011·Briefings in Bioinformatics·Melissa J HubiszAdam Siepel
Oct 28, 2011·Nature·Hyo Jung KangNenad Sestan
Jul 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Simone A FietzWieland B Huttner
Aug 11, 2012·Science·Santos J FrancoUlrich Müller
Aug 13, 2013·Molecular Cell·Shi-Yan NgLawrence W Stanton
Jan 28, 2014·Nature·Anamaria NecsuleaHenrik Kaessmann
Feb 14, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Caitlyn C GertzArnold R Kriegstein
Apr 4, 2014·Nature·Jeremy A MillerEd S Lein
May 29, 2014·Development·Marta Florio, Wieland B Huttner
Nov 19, 2014·PLoS Biology·Eric LewitusWieland B Huttner

❮ Previous
Next ❯

Citations

Jan 9, 2016·Molecular Biology and Evolution·Amir MarcovitzGill Bejerano
Nov 26, 2015·Fertility and Sterility·Eleni MantikouMark de Jong
Oct 31, 2015·Current Opinion in Neurobiology·Tracy L Young-Pearse, Eric M Morrow
Dec 8, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Debra L Silver
Nov 3, 2015·Nature Methods·Elliot R ThomsenSharad Ramanathan
Jan 23, 2016·Stem Cells International·Shuling ZhouHasan X Avci
Sep 26, 2015·Cell·Alex A PollenArnold R Kriegstein
Sep 12, 2015·Annual Review of Cell and Developmental Biology·Simona Lodato, Paola Arlotta
Aug 25, 2015·Cell Reports·Priya SrikanthTracy L Young-Pearse
Apr 16, 2016·Genome Biology·Siyuan John LiuDaniel A Lim
Apr 16, 2016·Genome Biology·Qing Ma, Howard Y Chang
May 27, 2016·Nature Neuroscience·Melanie D SweeneyBerislav V Zlokovic
Jun 4, 2016·Current Opinion in Neurobiology·Michaela Wilsch-BräuningerWieland B Huttner
Jun 4, 2016·Cell Stem Cell·Iva Kelava, Madeline A Lancaster
Jul 28, 2016·Neurobiology of Aging·Sarah M NeunerCatherine C Kaczorowski
Jul 2, 2016·BMC Systems Biology·Tom S WeberKen R Duffy
Oct 28, 2016·Cell Systems·Mauro J MuraroAlexander van Oudenaarden
Nov 9, 2016·Molecular and Cellular Neurosciences·Eoghan O'DuibhirSteven M Pollard
Jul 14, 2016·Stem Cells Translational Medicine·Kunie SakuraiWataru Fujibuchi
Jun 15, 2016·Antiviral Chemistry & Chemotherapy·Bryan D CoxRaymond F Schinazi
Feb 6, 2017·Human Molecular Genetics·Naoyuki MatsumotoHiroshi Kawasaki
Mar 5, 2017·Cell Stem Cell·Gerd Kempermann
Nov 5, 2016·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Xiaomin DongJia Qian Wu
Jun 22, 2017·Trends in Biotechnology·Ouriel CaenValerie Taly
Jul 18, 2017·Molecular Aspects of Medicine·Tallulah S Andrews, Martin Hemberg
Nov 18, 2017·Journal of the American Society for Mass Spectrometry·Sam B ChoiPeter Nemes
Nov 25, 2017·Frontiers in Neuroanatomy·Tatiana Popovitchenko, Mladen-Roko Rasin
Aug 30, 2018·Electrophoresis·Yue SunXingzhong Zhao
Jan 19, 2018·Proceedings of the National Academy of Sciences of the United States of America·Brian T KalishMichael E Greenberg
Apr 21, 2016·Nature Communications·Mayumi OkamotoAyano Kawaguchi
Aug 30, 2016·Nature Neuroscience·Charles G JenningsGuoping Feng
Aug 30, 2016·Nature Neuroscience·Jean-Francois PoulinRajeshwar Awatramani
May 10, 2019·American Journal of Medical Genetics. Part a·Kimia KahriziJennifer Winter

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Barrel cortex

Here is the latest research on barrel cortex, a region of somatosensory and motor corticies in the brain, which are used by animals that rely on whiskers for world exploration.

© 2022 Meta ULC. All rights reserved