Large-scale genetic perturbation screens are a classical approach in biology and have been crucial for many discoveries. New technologies can now provide unbiased quantification of multiple molecular and phenotypic changes across tens of thousands of individual cells from large numbers of perturbed cell populations simultaneously. In this Review, we describe how these developments have enabled the discovery of new principles of intracellular and intercellular organization, novel interpretations of genetic perturbation effects and the inference of novel functional genetic interactions. These advances now allow more accurate and comprehensive analyses of gene function in cells using genetic perturbation screens.
Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway
A large-scale overexpression screen in Saccharomyces cerevisiae identifies previously uncharacterized cell cycle genes
Zinc finger nucleases: custom-designed molecular scissors for genome engineering of plant and mammalian cells
Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile
Genomewide gain-of-function genetic screen identifies functionally active genes in mouse embryonic stem cells
Scoring diverse cellular morphologies in image-based screens with iterative feedback and machine learning.
Mass cytometry: technique for real time single cell multitarget immunoassay based on inductively coupled plasma time-of-flight mass spectrometry
A direct phenotypic comparison of siRNA pools and multiple individual duplexes in a functional assay
Patterns of basal signaling heterogeneity can distinguish cellular populations with different drug sensitivities
Actin and serum response factor transduce physical cues from the microenvironment to regulate epidermal stem cell fate decisions
The STRING database in 2011: functional interaction networks of proteins, globally integrated and scored
Cell-to-cell variability in PI3K protein level regulates PI3K-AKT pathway activity in cell populations
Systems-level analysis of microbial community organization through combinatorial labeling and spectral imaging
Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum
A haploid genetic screen identifies the major facilitator domain containing 2A (MFSD2A) transporter as a key mediator in the response to tunicamycin
Cell differentiation within a yeast colony: metabolic and regulatory parallels with a tumor-affected organism
Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway
A screen for morphological complexity identifies regulators of switch-like transitions between discrete cell shapes
Single-cell trajectory detection uncovers progression and regulatory coordination in human B cell development
Quantitative detection of rare interphase chromosome breaks and translocations by high-throughput imaging
An Unsupervised kNN Method to Systematically Detect Changes in Protein Localization in High-Throughput Microscopy Images
Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation
A metric and workflow for quality control in the analysis of heterogeneity in phenotypic profiles and screens
In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering
Transfer Learning with Deep Convolutional Neural Networks for Classifying Cellular Morphological Changes
Large-scale image-based profiling of single-cell phenotypes in arrayed CRISPR-Cas9 gene perturbation screens
Genomewide phenotypic analysis of growth, cell morphogenesis, and cell cycle events in Escherichia coli
Host factor prioritization for pan-viral genetic perturbation screens using random intercept models and network propagation
KCML: a machine-learning framework for inference of multi-scale gene functions from genetic perturbation screens.
From single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis
The Impact of CRISPR/Cas9 Technology on Cardiac Research: From Disease Modelling to Therapeutic Approaches
Identification of Parameters of Host Cell Vulnerability during Salmonella Infection by Quantitative Image Analysis and Modeling
Analysis of PARP inhibitor toxicity by multidimensional fluorescence microscopy reveals mechanisms of sensitivity and resistance
Systems-level interference strategies to decipher host factors involved in bacterial pathogen interaction: from RNAi to CRISPRi
Cortical structural differences in major depressive disorder correlate with cell type-specific transcriptional signatures.
CXCR4 Signaling Has a CXCL12-Independent Essential Role in Murine MLL-AF9-Driven Acute Myeloid Leukemia.
Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.
Alzheimer's Disease: MS4A
Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.
Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.
Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.
Genetic Screens in iPSC-derived Brain Cells
Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.
This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.
Chronic Fatigue Syndrome
Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.
Pharmacology of Proteinopathies
This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.
Alignment-free Sequence Analysis Tools
Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.