Single-cell biophysical study reveals deformability and internal ordering relationship in T cells.

Soft Matter
Blanca González-BermúdezGustavo R Plaza

Abstract

Deformability and internal ordering are key features related to cell function, particularly critical for cells that routinely undergo large deformations, like T cells during extravasation and migration. In the measurement of cell deformability, a considerable variability is typically obtained, masking the identification of possible interrelationships between deformability, internal ordering and cell function. We report the development of a single-cell methodology that combines measurements of living-cell deformability, using micropipette aspiration, and three-dimensional confocal analysis of the nucleus and cytoskeleton. We show that this single-cell approach can serve as a powerful tool to identify appropriate parameters that characterize deformability within a population of cells, not readably discernable in population-averaged data. By applying this single-cell methodology to mouse CD4+ T cells, our results demonstrate that the relative size of the nucleus, better than other geometrical or cytoskeletal features, effectively determines the overall deformability of the cells within the population.

References

Dec 3, 2005·Journal of Biomechanics·C T LimS T Quek
May 16, 2006·Nature Biotechnology·J Christopher LoveHidde L Ploegh
Mar 21, 2008·Progress in Biophysics and Molecular Biology·C CherubiniL Teresi
May 3, 2008·Nature·Tim LämmermannMichael Sixt
Aug 20, 2008·Nature Immunology·Peter Friedl, Bettina Weigelin
Dec 8, 2009·Science·Thomas D Pollard, John A Cooper
May 19, 2010·Cell·Steven J Altschuler, Lani F Wu
Sep 25, 2010·Nature Reviews. Immunology·Lydia Sorokin
Feb 2, 2013·Biomechanics and Modeling in Mechanobiology·Claudio M García-Herrera, Diego J Celentano
Aug 13, 2013·Biophysical Journal·Andrew G ClarkEwa K Paluch
Sep 7, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Janko Nikolich-Žugich
Dec 11, 2014·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·G R PlazaM Elices
Feb 11, 2015·The Journal of Cell Biology·William A ComrieJanis K Burkhardt
Dec 5, 2015·Frontiers in Immunology·Loïc DupréJavier Rey-Barroso
Feb 9, 2016·Nature Reviews. Immunology·Matthew F KrummelAudrey Gérard
Feb 16, 2016·Nature Immunology·Edward J CarrAdrian Liston
Apr 9, 2016·Trends in Immunology·Sussan NoursharghBeat A Imhof
May 15, 2016·Computer Methods in Biomechanics and Biomedical Engineering·Claudio M García-HerreraRoberto Ortega
Jun 1, 2016·The Journal of Cell Biology·Kenneth H Hu, Manish J Butte
Sep 7, 2016·European Journal of Immunology·Marcello PintiAndrea Cossarizza
Nov 5, 2016·Molecular Biology of the Cell·Lionel GuillouJulien Husson
Jan 18, 2017·Annals of Biomedical Engineering·Gustavo Esteban-ManzanaresGustavo R Plaza
Jan 20, 2017·Nature·Amos Tanay, Aviv Regev
Mar 9, 2017·Science Signaling·Timothy J ThaulandManish J Butte
May 23, 2017·Nature Cell Biology·Priyamvada ChughEwa K Paluch
Aug 2, 2017·Nature Methods·Marlon StoeckiusPeter Smibert
Sep 22, 2017·Molecular Biology of the Cell·Anna SawickaJulien Husson
Oct 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ming GuoDavid A Weitz
Dec 23, 2017·Nature Cell Biology·Sjoerd van HelvertPeter Friedl
Jun 20, 2018·Nature Methods·Pei-Hsun WuDenis Wirtz
Jul 31, 2018·Nature Reviews. Immunology·Jannie BorstWolfgang Kastenmüller
Sep 27, 2018·Molecular Biology of the Cell·Karthik DamodaranG V Shivashankar
Nov 22, 2018·Frontiers in Immunology·Nidhi SinhaJurjen Tel
Nov 30, 2018·Genome Biology·Robert J KimmerlingScott R Manalis
Jan 27, 2019·Biophysical Journal·Blanca González-BermúdezGustavo R Plaza
Jul 1, 2017·Nature Biomedical Engineering·Jude M PhillipDenis Wirtz

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Citations

Apr 4, 2021·Cells·Ashwini ShindeTuhin Subhra Santra
Jun 3, 2021·International Journal of Molecular Sciences·Maila ChirivìRoberto Rizzi

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Methods Mentioned

BETA
confocal microscopy
fluorescence imaging
rheology
flow cytometry
confocal
super-resolution microscopy

Software Mentioned

ImageJ 3D Plugins
MATLAB
LabVIEW
Fytik
ImageJ
AutoCAD

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