Single-cell characterization of metabolic switching in the sugar phosphotransferase system of Escherichia coli

Molecular Microbiology
Sonja A WestermayerJoachim O Rädler

Abstract

The utilization of several sugars in Escherichia coli is regulated by the Phosphotransferase System (PTS), in which diverse sugar utilization modules compete for phosphoryl flux from the general PTS proteins. Existing theoretical work predicts a winner-take-all outcome when this flux limits carbon uptake. To date, no experimental work has interrogated competing PTS uptake modules with single-cell resolution. Using time-lapse microscopy in perfused microchannels, we analyzed the competition between N-acetyl-glucosamine and sorbitol, as representative PTS sugars, by measuring both the expression of their utilization systems and the concomitant impact of sugar utilization on growth rates. We find two distinct regimes: hierarchical usage of the carbohydrates, and co-expression of the genes for both systems. Simulations of a mathematical model incorporating asymmetric sugar quality reproduce our metabolic phase diagram, indicating that under conditions of nonlimiting phosphate flux, co-expression is due to uncoupling of both sugar utilization systems. Our model reproduces hierarchical winner-take-all behaviour and stochastic co-expression, and predicts the switching between both strategies as a function of available phosphate flux. ...Continue Reading

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Citations

Dec 1, 2017·Journal of the Royal Society, Interface·Hidde de JongJean-Luc Gouzé
May 7, 2020·Pflügers Archiv : European journal of physiology·Jean-Marc Jeckelmann, Bernhard Erni
Dec 31, 2020·Proceedings of the National Academy of Sciences of the United States of America·Tamar SzokeOrna Amster-Choder
Nov 24, 2020·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Andreas E Vasdekis, Abhyudai Singh
Jul 3, 2021·Nutrients·Kouya HattoriYun-Gi Kim

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