Single cell electroporation method for axon tracing in cultured slices

Development, Growth & Differentiation
Naofumi UesakaNobuhiko Yamamoto

Abstract

Axon tracing is an essential method to reveal neuronal connection patterns and characteristics of growing axons during development. Here we introduce an electroporation-based gene transfer technique with a fluorescent protein (FP), which enables us to observe a small number of axons. The result also demonstrates that dynamics of axon behavior can be followed for more than a week. Thus, such labeling with FP is a powerful tool for axon tracing.

References

Jun 20, 2003·Journal of Neuroscience Methods·I SahlyMicha E Spira
Jan 7, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Naofumi UesakaNobuhiko Yamamoto

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Citations

May 25, 2010·Analytical and Bioanalytical Chemistry·Manyan WangStephen G Weber
Nov 26, 2010·The Journal of Membrane Biology·Oksana DyachokTerence F McDonald
Apr 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Akito YamadaNobuhiko Yamamoto
Nov 9, 2010·International Archives of Medicine·Ti-Fei YuanOscar Arias-Carrión
Apr 27, 2012·PloS One·Joseph D Steinmeyer, Mehmet Fatih Yanik
Jun 11, 2015·Developmental Neurobiology·Naoyuki MatsumotoNobuhiko Yamamoto
Apr 5, 2013·Development, Growth & Differentiation·Hiromu YawoToru Ishizuka
Jun 27, 2020·Cells·Pallavi GuptaTuhin Subhra Santra
Dec 18, 2020·Frontiers in Neural Circuits·Sayaka SugiyamaXubin Hou

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