PMID: 16627992Apr 22, 2006Paper

Single cell FRET imaging for determination of pathway of tumor cell apoptosis induced by photofrin-PDT

Cell Cycle
Yun-Xia WuWei R Chen

Abstract

Apoptosis is an important cellular event that plays a key role in pathogeny and therapy of many diseases. Apoptosis has been associated with photodynamic therapy (PDT) and its pathway is important in the mechanistic study of PDT. We show that single cell fluorescent imaging can be used to determine the pathway of PDT-induced tumor cell apoptosis. In this study, ASTC-a-1 tumor cells transfected by plasmid DNA SCAT3 were treated by Photofrin-PDT. The intracellular distribution of Photofrin was observed using a confocal microscope. The activations of caspase-3 and caspase-8 were dynamically observed using fluorescence resonance energy transfer (FRET). Our experimental results show that the Photofrin molecules are localized in cell mitochondria, and that after PDT caspase-3 was activated rapidly while caspase-8 remained inactive. These results demonstrate that the tumor cell apoptosis induced by Photofrin-PDT was directly initiated from the mitochondrial pathway.

Citations

May 24, 2007·Apoptosis : an International Journal on Programmed Cell Death·Yihui PeiTongsheng Chen
Oct 3, 2012·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Huali LiTongsheng Chen
Jan 5, 2008·Photochemistry and Photobiology·Qingling WanQun Chen
Dec 25, 2012·Neuroscience Letters·Harumasa NakazawaTakehiko Iijima
Aug 4, 2009·Micron : the International Research and Review Journal for Microscopy·Longxiang WangXunbin Wei
Jun 3, 2008·Micron : the International Research and Review Journal for Microscopy·Xiao-Ping WangMartin Mok
May 1, 2010·Journal of Cellular Physiology·Shiming LuoQun Chen
Nov 18, 2009·Journal of Cellular Physiology·Qingrui SunXunbin Wei
May 15, 2007·Biochemical and Biophysical Research Communications·Miaojuan ChenLan Zhang
Sep 15, 2007·Cellular Signalling·Yinyuan WuXichao Wang
Aug 19, 2007·Biochimica Et Biophysica Acta·Esther BuytaertPatrizia Agostinis
Apr 30, 2016·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Ayumi KonnoTakehiko Iijima
Jul 29, 2010·Physiological Reviews·Dmitriy M ChudakovKonstantin A Lukyanov
Oct 29, 2015·Methods and Applications in Fluorescence·Clare E RowlandJames B Delehanty
Jun 12, 2019·Bioinorganic Chemistry and Applications·Nthabeleng HlapisiSandile P Songca
Jul 6, 2020·Apoptosis : an International Journal on Programmed Cell Death·Zhijing XuFu Wang

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