Abstract
Single-cell gel electrophoresis, or the comet assay, a technique widely used for DNA damage analysis, has been used recently for detecting DNA fragmentation in cells undergoing apoptosis. However, the number of variants of this assay used thus far primarily detected the late stages of DNA fragmentation. Therefore, monitoring the progression of DNA fragmentation, which could greatly improve the analysis of cell death induction and progression at the single-cell level, has not been possible with this assay. In the present study, a modification of the original neutral comet assay developed by Ostling and Johanson (Biochem Biophys Res Commun 123:291-298, 1984) was used to detect various stages of DNA fragmentation. This assay involves cell lysis with anionic detergents at nearly neutral pH (9.5) and does not include high salt concentration, unlike most other published methods. BMG-1 human glioma cells were induced to undergo programmed cell death by treating with a large dose (100 microM) of etoposide, and comets were prepared after different durations (1-24 h) of treatment. In contrast to results of previously published studies, comets with different shapes reflecting progressive stages of DNA fragmentation were observed. Of these...Continue Reading
References
Nov 1, 1989·International Journal of Radiation Oncology, Biology, Physics·B S Dwarkanath, V K Jain
Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Aug 30, 1984·Biochemical and Biophysical Research Communications·O Ostling, K J Johanson
Nov 1, 1995·Experimental Cell Research·P L Olive, J P Banáth
Feb 1, 1995·Journal of Biochemical and Biophysical Methods·P Gopalakrishna, A Khar
Jul 1, 1993·Mutation Research·V J McKelvey-MartinA Collins
Mar 1, 1995·In Vitro Cellular & Developmental Biology. Animal·D W Fairbairn, K L O'Neill
Feb 1, 1995·Mutation Research·D W FairbairnK L O'Neill
Jan 15, 1994·FEMS Microbiology Letters·D W FairbairnK L O'Neill
Aug 25, 1999·Histochemistry and Cell Biology·T GodardP Gauduchon
Nov 26, 1999·BioTechniques·N KizilianJ P McNamee
Jun 19, 2001·Mutation Research·P ChoucrounJ D Dewitte
Jun 14, 2002·Biochemical and Biophysical Research Communications·Stefan CzeneMats Harms-Ringdahl
Jul 13, 2002·Cytometry·Ruth C WilkinsJ R N McLean
Sep 5, 2002·Free Radical Biology & Medicine·Alexandra BarboutiDimitrios Galaris
Jun 18, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Shingo YasuharaJ A Jeevendra Martyn
Citations
Sep 29, 2011·Cytotechnology·Shubhankar SumanSudhir Chandna
Dec 4, 2008·Investigational New Drugs·Daniéli GerhardtChristianne Salbego
Jan 1, 1992·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·R M StewartD R Morrison
May 3, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Laura BanduraWlodzimierz Korohoda
Oct 14, 2014·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Marzena Szwed, Zofia Jozwiak
Sep 13, 2011·Toxicology and Applied Pharmacology·Satyender SinghArvind Rai
Feb 5, 2011·Toxicology and Applied Pharmacology·Satyender SinghArvind Rai
Jul 27, 2011·Environmental Toxicology and Pharmacology·Satyender SinghArvind Rai
Jan 17, 2009·The International Journal of Biochemistry & Cell Biology·Regalla KumarswamySudhir Chandna
Aug 16, 2005·Cell Proliferation·Xuan HuangZbigniew Darzynkiewicz
Nov 16, 2007·Journal of Cellular Biochemistry·Milena GeorgievaGeorge Miloshev
Mar 9, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Ozge Gursoy-YuzugulluMehmet Ozturk
Apr 30, 2015·Journal of Investigative and Clinical Dentistry·Swetha PauloseSivakumar Brooklyin
Jun 19, 2013·Free Radical Research·R Åsgård, B Hellman
Oct 20, 2012·International Journal of Radiation Biology·Manish AdhikariRajesh Arora
Jul 19, 2013·International Journal of Radiation Biology·Sudhir ChandnaRakesh Kumar Seth
Jun 13, 2015·Journal of Photochemistry and Photobiology. B, Biology·Moirangthem Kameshwor SinghRina Chakrabarti
May 23, 2012·Radiation Research·Ammar H J Al-MayahMunira A Kadhim
Jul 9, 2011·Mutation Research·Satyender SinghArvind Rai
Nov 5, 2014·PloS One·Vijay SinghRoger M Macklis
May 1, 2015·BMC Cancer·Anant Narayan BhattBilikere S Dwarakanath
Feb 18, 2017·Journal of Endocrinological Investigation·C FantiniL Di Luigi
Dec 14, 2017·Molecular and Cellular Biochemistry·Jyoti Swaroop KumarSudhir Chandna
May 5, 2009·Biotechnology and Applied Biochemistry·Pankaj Kumar SinghRakesh Kumar Sharma
May 22, 2010·Cell Biology International·Regalla Kumarswamy, Sudhir Chandna
Jul 17, 2015·Electrophoresis·Milena GeorgievaGeorge Miloshev
Nov 18, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·R ChakrabartiP Mittal
Jul 11, 2007·Molecular and Cellular Biochemistry·Urmila TawarVibha Tandon
Dec 27, 2005·Carcinogenesis·Craig G MoneypennyEvan P Gallagher
Nov 14, 2020·International Journal of Molecular Sciences·Seda Tuncay CagatayMunira Kadhim