Single-Cell Genomics Reveals a Diverse Metabolic Potential of Uncultivated Desulfatiglans -Related Deltaproteobacteria Widely Distributed in Marine Sediment

Frontiers in Microbiology
Lara M JochumKasper Urup Kjeldsen

Abstract

Desulfatiglans-related organisms comprise one of the most abundant deltaproteobacterial lineages in marine sediments where they occur throughout the sediment column in a gradient of increasing sulfate and organic carbon limitation with depth. Characterized Desulfatiglans isolates are dissimilatory sulfate reducers able to grow by degrading aromatic hydrocarbons. The ecophysiology of environmental Desulfatiglans-populations is poorly understood, however, possibly utilization of aromatic compounds may explain their predominance in marine subsurface sediments. We sequenced and analyzed seven Desulfatiglans-related single-cell genomes (SAGs) from Aarhus Bay sediments to characterize their metabolic potential with regard to aromatic compound degradation and energy metabolism. The average genome assembly size was 1.3 Mbp and completeness estimates ranged between 20 and 50%. Five of the SAGs (group 1) originated from the sulfate-rich surface part of the sediment while two (group 2) originated from sulfate-depleted subsurface sediment. Based on 16S rRNA gene amplicon sequencing group 2 SAGs represent the more frequent types of Desulfatiglans-populations in Aarhus Bay sediments. Genes indicative of aromatic compound degradation could be...Continue Reading

References

Apr 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·M C EvansD I Arnon
Aug 30, 1994·Biochimica Et Biophysica Acta·J S LolkemaW N Konings
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·O DymD Eisenberg
Aug 31, 2000·Journal of Molecular Biology·C NotredameJ Heringa
Jul 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·F O GlöcknerR Reinhardt
Feb 27, 2004·Nucleic Acids Research·Wolfgang LudwigKarl-Heinz Schleifer
Sep 4, 2004·Applied and Environmental Microbiology·Tina HölscherLorenz Adrian
May 1, 1980·Applied and Environmental Microbiology·G M King, M J Klug
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jennifer F BiddleKai-Uwe Hinrichs
Apr 25, 2007·IUBMB Life·Aurelio SerranoHerrick Baltscheffsky
May 8, 2008·Nature Reviews. Microbiology·Gerard Muyzer, Alfons J M Stams
Sep 20, 2008·Biochimica Et Biophysica Acta·Stephen W Ragsdale, Elizabeth Pierce
Oct 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michael Richter, Ramon Rosselló-Móra
Jul 30, 2010·The ISME Journal·Achim QuaiserPurificación López-García
Jan 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Anne-Kristin KasterRudolf K Thauer
Mar 11, 2011·Journal of Chemical Information and Modeling·Claudia AndreiniAntonio Rosato
Jun 8, 2011·Microbiology and Molecular Biology Reviews : MMBR·Beth N OrcuttKatrina J Edwards
Jul 8, 2011·Frontiers in Microbiology·Caroline M PluggeAlfons J M Stams
Jul 12, 2011·Applied and Environmental Microbiology·Dawn E HolmesDerek R Lovley
Jul 13, 2011·Frontiers in Microbiology·Inês A Cardoso PereiraSofia Santos Venceslau
Oct 4, 2011·Nature Reviews. Microbiology·Georg FuchsJohann Heider
Feb 22, 2012·Frontiers in Microbiology·Mark Alexander Lever
Apr 18, 2012·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Anton BankevichPavel A Pevzner

❮ Previous
Next ❯

Citations

Jan 4, 2020·The ISME Journal·Peng PengSiavash Atashgahi
Oct 2, 2020·Environmental Microbiology·Daan M van VlietIrene Sánchez-Andrea
Feb 16, 2021·Environmental Microbiology·Peter Q FischerDiana Z Sousa
Jun 24, 2021·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey J MarlowPeter R Girguis
Aug 1, 2021·The ISME Journal·Marguerite V LangwigBrett J Baker
Jan 4, 2022·Environmental Science & Technology·Caroline E PierceBrandy M Toner

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA308429
PRJNA377833

Methods Mentioned

BETA
density gradient centrifugation
PCR
amplicon sequencing

Software Mentioned

blastp
trimmomatic
enveomics
CheckM
MetalPredator
RAxML
ILLUMINACLIP
PROTGAMMALGF
GTRGAMMA
ARB

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.