Single-cell immune checkpoint landscape of PBMCs stimulated with Candida albicans.

Emerging Microbes & Infections
Weiwei DengRuoyu Li

Abstract

Immune checkpoints play various important roles in tumour immunity, which usually contribute to T cells' exhaustion, leading to immunosuppression in the tumour microenvironment. However, the roles of immune checkpoints in infectious diseases, especially fungal infection, remain elusive. Here, we reanalyzed a recent published single-cell RNA-sequencing (scRNA-seq) data of peripheral blood mononuclear cells (PBMCs) stimulated with Candida albicans (C. albicans), to explore the expression patterns of immune checkpoints after C. albicans bloodstream infection. We characterized the heterogeneous pathway activities among different immune cell subpopulations after C. albicans infection. The CTLA-4 pathway was up-regulated in stimulated CD4+ and CD8+ T cells, while the PD-1 pathway showed high activity in stimulated plasmacytoid dendritic cell (pDC) and monocytes. Importantly, we found that immunosuppressive checkpoints HAVCR2 and LAG3 were only expressed in stimulated NK and CD8+ T cells, respectively. Their viabilities were validated by flow cytometry. We also identified three overexpressed genes (ISG20, LY6E, ISG15) across all stimulated cells. Also, two monocyte-specific overexpressed genes (SNX10, IDO1) were screened out in this s...Continue Reading

References

Jun 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dennis M LindellGary B Huffnagle
Nov 16, 2005·Nature Immunology·Chen ZhuVijay K Kuchroo
Dec 31, 2008·BMC Bioinformatics·Peter Langfelder, Steve Horvath
Nov 19, 2010·Current Topics in Microbiology and Immunology·Monica V Goldberg, Charles G Drake
Jul 9, 2011·Nature Immunology·E John Wherry
Mar 14, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·David R AndesUNKNOWN Mycoses Study Group
Dec 20, 2012·Mucosal Immunology·N Hernández-SantosS L Gaffen
Jul 16, 2013·PLoS Pathogens·Antonella De LucaLuigina Romani
Nov 5, 2014·Nature·Yu-Hwa HuangRichard S Blumberg
Dec 22, 2016·The New England Journal of Medicine·Xavier MontalbanUNKNOWN ORATORIO Clinical Investigators
Mar 5, 2017·Immunological Reviews·Lawrence P AndrewsDario A A Vignali
May 12, 2017·PloS One·Francesco BarchiesiEsther Manso
Jun 15, 2017·Journal of Molecular Endocrinology·Thalijn Liliana Catharina WoltersRomana Teodora Netea-Maier
Sep 16, 2017·Pathogens and Disease·María Soledad MiróClaudia Elena Sotomayor
Oct 11, 2017·Nature Reviews. Immunology·Michelle N Wykes, Sharon R Lewin
Nov 8, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna WasiukLi-Zhen He
Aug 10, 2018·Frontiers in Microbiology·Qi Hui SamLouis Yi Ann Chai
Nov 6, 2018·Molecular Therapy : the Journal of the American Society of Gene Therapy·Diogo Gomes-SilvaMaksim Mamonkin
Jan 4, 2019·Nature Communications·Maksim KunitskiReinhard Dörner
Jul 23, 2019·Seminars in Cancer Biology·Shaheen Khan, David E Gerber
Aug 23, 2019·Nature Communications·Zhengtao XiaoJason W Locasale
Sep 9, 2019·Trends in Molecular Medicine·Michael S AbersDimitrios P Kontoyiannis
Sep 21, 2019·The Journal of Experimental Medicine·Rémy RodriguezSylvain Latour
Sep 30, 2018·Journal of Infection in Developing Countries·Jianwei LiuJie Zhang
Aug 1, 2020·Frontiers in Cellular and Infection Microbiology·Orchi DuttaAmariliz Rivera

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.