Single cell immune profiling of dengue virus patients reveals intact immune responses to Zika virus with enrichment of innate immune signatures.

PLoS Neglected Tropical Diseases
Yujiao ZhaoRuth R Montgomery

Abstract

The genus Flavivirus contains many mosquito-borne human pathogens of global epidemiological importance such as dengue virus, West Nile virus, and Zika virus, which has recently emerged at epidemic levels. Infections with these viruses result in divergent clinical outcomes ranging from asymptomatic to fatal. Myriad factors influence infection severity including exposure, immune status and pathogen/host genetics. Furthermore, pre-existing infection may skew immune pathways or divert immune resources. We profiled immune cells from dengue virus-infected individuals by multiparameter mass cytometry (CyTOF) to define functional status. Elevations in IFNβ were noted in acute patients across the majority of cell types and were statistically elevated in 31 of 36 cell subsets. We quantified response to in vitro (re)infection with dengue or Zika viruses and detected a striking pattern of upregulation of responses to Zika infection by innate cell types which was not noted in response to dengue virus. Significance was discovered by statistical analysis as well as a neural network-based clustering approach which identified unusual cell subsets overlooked by conventional manual gating. Of public health importance, patient cells showed signifi...Continue Reading

References

Feb 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Adeline R WhitneyPatrick O Brown
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Mar 24, 2007·Annual Review of Immunology·Andrew D HislopAlan B Rickinson
Apr 25, 2007·The Journal of Experimental Medicine·Kerstin LühnSarah Rowland-Jones
Sep 21, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Duane J Gubler
Aug 13, 2008·Cytokine·Shohei KoyamaShizuo Akira
Aug 30, 2008·The Journal of Infectious Diseases·Michael P BuschSally Caglioti
Mar 17, 2009·Cadernos de saúde pública·Héctor Gómez-Dantés, Janine Ramsey Willoquet
Jun 12, 2009·The New England Journal of Medicine·Mark R DuffyEdward B Hayes
Sep 16, 2009·Annals of the New York Academy of Sciences·Scott B Halstead
Jan 12, 2011·Annual Review of Immunology·Brian R Murphy, Stephen S Whitehead
Apr 8, 2011·Cytometry. Part B, Clinical Cytometry·Debora R SekiguchiEline T Luning Prak
Jul 12, 2011·Nature Immunology·Helder I NakayaBali Pulendran
Jul 28, 2011·Cellular Microbiology·Alvaro ArjonaErol Fikrig
Jan 6, 2012·Acta Crystallographica. Section E, Structure Reports Online·Zhiliang Wang, Xianju Zhou
Feb 22, 2012·Nature Reviews. Immunology·Holden T MaeckerRobert Nussenblatt
Apr 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ruklanthi de AlwisAravinda M de Silva
Apr 14, 2012·Tropical Medicine and Health·Nguyen Thi Phuong Lan, Kenji Hirayama
May 30, 2012·Epidemiology and Infection·L R PetersenM P Busch
Oct 5, 2012·Clinical Microbiology Reviews·Tonya M ColpittsErol Fikrig
Feb 5, 2013·Seminars in Immunology·Arnon AraziNir Hacohen
Apr 9, 2013·Nature·Samir BhattSimon I Hay
Apr 27, 2013·Science·Amanda M JamiesonRuslan Medzhitov
Jan 21, 2014·Nature Biotechnology·Vladimir BrusicUNKNOWN HIPC steering committee
Mar 13, 2014·Viral Immunology·Feng QianRuth R Montgomery
Sep 19, 2014·Lancet·Maria G Guzman, Eva Harris
Oct 31, 2014·Clinical and Vaccine Immunology : CVI·Feng QianRuth R Montgomery
Dec 3, 2014·Journal of Immunological Methods·Yi YaoRuth R Montgomery
Feb 28, 2015·Current Opinion in Immunology·Keven M RobinsonJohn F Alcorn
Jul 24, 2015·Science Translational Medicine·Dara M Strauss-AlbeeCatherine A Blish
Sep 25, 2015·Emerging Infectious Diseases·Gubio S CamposSilvia I Sardi
Jan 29, 2016·MMWR. Morbidity and Mortality Weekly Report·Lavinia Schuler-FacciniUNKNOWN Brazilian Medical Genetics Society–Zika Embryopathy Task Force
Feb 11, 2016·The New England Journal of Medicine·Jernej MlakarTatjana Avšič Županc
Feb 28, 2016·BMJ : British Medical Journal·Marina BasarabIan Cropley
Mar 26, 2016·Science·Nuno Rodrigues FariaPedro F C Vasconcelos
Mar 31, 2016·The New England Journal of Medicine·Rita W DriggersOlli Vapalahti
May 24, 2016·Cell Host & Microbe·Alesha GrantAdolfo García-Sastre
Jun 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Lalita PriyamvadaJens Wrammert

❮ Previous
Next ❯

Citations

Sep 11, 2020·Journal of Leukocyte Biology·Emma StewartRuth R Montgomery
Nov 3, 2020·Clinical and Translational Medicine·Ting ZhangXianting Ding

❮ Previous
Next ❯

Datasets Mentioned

BETA
SDY1369

Methods Mentioned

BETA
ELISA
density-gradient centrifugation
flow cytometry
FACS
FCS

Software Mentioned

R
Cytobank
SAS
FlowJo
SAUCIE
tidyverse
SAUCIE ( Sparse Autoencoder for Unsupervised Clustering , Impu...
Normalizer
CyTOF

Related Concepts

Related Feeds

CZI Human Cell Atlas Seed Network

The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.

Related Papers

The Journal of Infectious Diseases
Anna B Kawiecki, Rebecca C Christofferson
MMWR. Morbidity and Mortality Weekly Report
Ingrid B RabeAnn M Powers
MMWR. Recommendations and Reports : Morbidity and Mortality Weekly Report. Recommendations and Reports
Tyler M SharpStephen H Waterman
© 2021 Meta ULC. All rights reserved