PMID: 6989382Feb 1, 1980Paper

Single-cell immunofluorescence assay for terminal transferase: human leukaemic and non-leukaemic cells

British Journal of Cancer
S OkamuraT W Mak

Abstract

The characteristics of a single-cell immunofluorescence assay for terminal deoxynucleotidyl transferase (terminal transferase, TdT) is described. The data indicate that the single-cell immunofluorescence assay is highly efficient and specific for the detection of cells containing TdT. Using this assay, we have examined 124 marrow or peripheral-blood samples from 104 patients with or without haematological malignancies. Results indicate that TdT(+) cells from 6% to 100% were found in the following patients: 34/40 samples from patients with ALL at the time of diagnosis or during relapse; 2/3 patients with acute undifferentiated leukaemia; 2/3 patients with acute myelomonocytic leukaemia; 1/24 patients with acute myeloblastic leukaemia; 1/5 patients with chronic myelocytic leukaemia (CML) in blastic crisis; and 2/2 patients with diffuse lymphoblastic lymphoma. In contrast less than 1% of TdT(+) cells were found in 20 marrow or peripheral-blood samples from ALL patients in complete remission; 8 patients with CML in chronic phase; 2 patients with myeloma; 1 sample from a patient with Hodgkin's disease, peripheral-blood samples from 7 normal donors and marrow samples from 6 patients without haematological malignancies. TdT(+) cells w...Continue Reading

Citations

Jan 1, 1992·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·K ShimodaY Niho
Apr 1, 1991·European Journal of Haematology·S KondoY Niho
Jan 1, 1988·European Journal of Haematology·Y HirotaY Niho
Jan 1, 1988·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·N OhharaY Niho
Apr 1, 1983·Scandinavian Journal of Haematology·H J van der ReijdenA E von dem Borne

Related Concepts

Marrow
Tdt Antigen
DNA Nucleotidyltransferases
Immunofluorescence Assay
Leukemia
Lymphoid Leukemia
Lymphoid Cells

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