Single cell level detection of Escherichia coli in microfluidic device

Biosensors & Bioelectronics
Jin-Hee HanJeong-Yeol Yoon

Abstract

Detection of Escherichia coli K-12 in phosphate buffered saline (PBS) was demonstrated in a Y-channel polydimethylsiloxane (PDMS) microfluidic device through optical fiber monitoring of latex immunoagglutination. The latex immunoagglutination assay was performed for serially diluted E. coli solutions using 0.92-microm highly carboxylated polystyrene particles conjugated with polyclonal anti-E. coli. Pre-treatments such as cell lysis or culturing to enhance the signal were not used. Proximity optical fibers around the view cell of the device were used to quantify the increase in 45 degrees forward light scattering of the immunoagglutinated particles. In order to reduce false positive signals caused by antibodies binding to non-viable E. coli cells or free antigens in solution, target solutions were washed three times, and then the results were compared to non-washing treatments. The detection limit was found to be less than 10 cfu ml(-1) (1 cfu per device) without PBS washing (thus detecting non-viable cells and free antigens), or less than 40 cfu ml(-1) (4 cfu per device) with PBS washing (thus detecting viable E. coli cells only).

Citations

Jun 16, 2010·Analytical and Bioanalytical Chemistry·Iuliana OitaDebby Mangelings
Oct 5, 2010·Journal of Environmental Monitoring : JEM·Hyuck-Jin KwonJeong-Yeol Yoon
Nov 19, 2008·Journal of Biological Engineering·Jeong-Yeol Yoon, David J You
Sep 24, 2009·Journal of Biological Engineering·Jeong-Yeol Yoon, Mark R Riley
Apr 30, 2009·Journal of Biological Engineering·Jin-Hee Han, Jeong-Yeol Yoon
Nov 1, 2012·Sensors·Jeong-Yeol Yoon, Bumsang Kim
Aug 31, 2011·International Journal of Nephrology·Olivia Boyer, Patrick Niaudet
Oct 23, 2010·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Jun TeramotoAkira Ishihama
Aug 7, 2012·Biosensors & Bioelectronics·David J YouJeong-Yeol Yoon
Sep 4, 2012·Biosensors & Bioelectronics·Christopher F FronczekJeong-Yeol Yoon
Mar 19, 2014·Water Research·Helen BridleMarc P Y Desmulliez
Jul 6, 2013·Biosensors & Bioelectronics·Yu-Tzu ChenJing-Tang Yang
Jul 15, 2015·Biosensors & Bioelectronics·Scott V AngusJeong-Yeol Yoon
Jul 21, 2015·Biosensors & Bioelectronics·Soohee ChoJeong-Yeol Yoon
Jan 1, 2009·Sensors·Wanida LaiwattanapaisalAdisorn Tuantranont
Jun 12, 2014·Annals of Biomedical Engineering·Xiyuan LiuPeter B Lillehoj
Oct 29, 2013·Lab on a Chip·Tu San ParkJeong-Yeol Yoon

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