Single-cell level response of HIV-specific and cytomegalovirus-specific CD4 T cells correlate with viral control in chronic HIV-1 subtype A infection

Journal of Acquired Immune Deficiency Syndromes : JAIDS
Michael A EllerJeffrey R Currier

Abstract

HIV-1 subtype A is the second most prevalent subtype globally and is associated with reduced viral load, higher CD4 absolute counts, and slower disease progression. To study the possible role of T cells associated with better outcome, we examined CD4 and CD8 T-cell responses against HIV-1 and cytomegalovirus (CMV) in Ugandans infected with subtype A HIV-1. T-cell responses were investigated using flow cytometry and novel subtype A variant inclusive peptide (VIP) sets designed for this evaluation. CD4 T-cell responses focused primarily on Gag, whereas CD8 T-cell responses were broadly directed against Gag, gp41, and Nef VIP sets. CD4 T cells primarily responded with interferon (IFN)-γ, whereas CD8 cells were more diverse with degranulation (CD107a), IFN-γ, and macrophage inflammatory protein (MIP)-1β production. No relationship was observed between CD8 T-cell responses and the HIV-1 load. Similarly, the frequency of CD4 T cells responding to these antigens did not associate with viral control. However, in CD4 T cells responding against Gag or CMV, the IFN-γ intensity, indicative of the production at the single-cell level, was inversely proportional to viral load. No significant relationship was found between T-cell effector/memo...Continue Reading

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Citations

Apr 19, 2017·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Rita CasettiChiara Agrati
Jun 27, 2015·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Christian PrebensenDag Kvale
Jan 23, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kim BlomJohan K Sandberg

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