Single-cell mapping of neural and glial gene expression in the developing Drosophila CNS midline cells.

Developmental Biology
Scott R WheelerS T Crews

Abstract

Understanding the generation of neuronal and glial diversity is one of the major goals of developmental neuroscience. The Drosophila CNS midline cells constitute a simple neurogenomic system to study neurogenesis, cell fate acquisition, and neuronal function. Previously, we identified and determined the developmental expression profiles of 224 midline-expressed genes. Here, the expression of 59 transcription factors, signaling proteins, and neural function genes was analyzed using multi-label confocal imaging, and their expression patterns mapped at the single-cell level at multiple stages of CNS development. These maps uniquely identify individual cells and predict potential regulatory events and combinatorial protein interactions that may occur in each midline cell type during their development. Analysis of neural function genes, including those encoding peptide neurotransmitters, neurotransmitter biosynthetic enzymes, transporters, and neurotransmitter receptors, allows functional characterization of each neuronal cell type. This work is essential for a comprehensive genetic analysis of midline cell development that will likely have widespread significance given the high degree of evolutionary conservation of the genes analy...Continue Reading

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Citations

Mar 14, 2008·PLoS Biology·Irene Miguel-AliagaAlex P Gould
Nov 12, 2009·BMC Developmental Biology·Scott R WheelerStephen T Crews
Nov 10, 2010·Development Genes and Evolution·Tatiana MorozovaMargaret Sonnenfeld
Aug 3, 2012·Nucleic Acids Research·Gilbert L HenrySean R Eddy
Dec 2, 2015·Molecular and Cellular Neurosciences·Shingo YoshikawaJohn B Thomas
Sep 29, 2011·Gene Expression Patterns : GEP·Stephanie M FreerStephen T Crews
Jan 21, 2009·Current Opinion in Genetics & Development·Todd H Oakley, Ajna S Rivera
Aug 13, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kirsten A GussJames B Skeath
Feb 8, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kirsten A GussJames B Skeath
Dec 15, 2010·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Eric Fulkerson, Patricia Ann Estes
Sep 15, 2015·Brain Research·Stefanie SchirmeierChristian Klämbt
Nov 9, 2011·Developmental Biology·Scott R WheelerStephen T Crews
Dec 17, 2009·Cell Adhesion & Migration·Stephen T Crews
Dec 9, 2008·Current Biology : CB·Mikyung V ChangKen M Cadigan
May 24, 2014·Developmental Biology·Joseph C Pearson, Stephen T Crews
Dec 3, 2014·Frontiers in Neuroscience·Stefanie LimmerChristian Klämbt
Jun 28, 2016·Developmental Biology·Kushal SuryamohanMarc S Halfon
Sep 21, 2007·Endocrine Reviews·Mette Christine JørgensenJacob Hecksher-Sørensen
Nov 18, 2018·Glia·Kerem YildirimChristian Klämbt
Aug 10, 2012·Development·Joseph D Watson, Stephen T Crews
Jul 17, 2021·Cells & Development·Jessica C YuRodrigo Fernandez-Gonzalez

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