Single-cell measurements of purine release using a micromachined electroanalytical sensor

Analytical Chemistry
C D BrattenJ M Cooper

Abstract

To study the cellular events surrounding the formation of purines in cardiac ischemia, we have micromachined a micrometer-scale titer chamber containing an integrated electrochemical sensor, capable of measuring analytes produced by a single heart cell. The analytical procedure involves the determination of metabolites via the amperometric detection of enzymically generated hydrogen peroxide, measured at a platinized microelectrode, poised at a suitably oxidizing potential, equivalent to +420 mV vs Ag/AgCl. Signals were recorded as current-time responses and were integrated to give a total charge (Q) attributable to the reaction under investigation. The amount of analyte produced by the cell was subsequently quantified by the addition of a known amount of calibrant. As a consequence, by using a cascade of three enzymes (adenosine deaminase, nucleotide phosphorylase, and xanthine oxidase), we were able to show that, after rigor contracture had been induced in a single myocyte, adenosine (but not inosine) only reached the extracellular space after the cell membrane had been permeabilized by detergent. These data, which could only be obtained unambiguously by using this single-cell methodology, have provided us with information on...Continue Reading

References

Jul 1, 1992·The American Journal of Physiology·G GstraunthalerW Pfaller
May 1, 1991·The American Journal of Physiology·J G PickarR C Carlsen
Feb 1, 1987·Circulation Research·D G Allen, C H Orchard
Jun 1, 1987·The Biochemical Journal·A AllshireP H Cobbold
Mar 1, 1972·The American Journal of Physiology·V R RubioR M Berne
Aug 1, 1966·Circulation Research·M Katori, R M Berne
Dec 1, 1980·Circulation Research·R M Berne
May 1, 1984·Circulation Research·T GeisbuhlerG P Brierley
Mar 10, 1995·The Journal of Biological Chemistry·J M Finnegan, R M Wightman

❮ Previous
Next ❯

Citations

Nov 23, 2006·Analytical and Bioanalytical Chemistry·Jeffrey S LenihanLeonidas G Bachas
Apr 19, 2005·Biomedical Microdevices·Kwang-Seok Yun, Euisik Yoon
Jan 25, 2008·Biomedical Microdevices·Igor A GesFranz J Baudenbacher
Jul 19, 2008·Annual Review of Analytical Chemistry·Ivar Meyvantsson, David J Beebe
Nov 10, 2001·Annual Review of Biomedical Engineering·J VoldmanM A Schmidt
Mar 23, 2011·Analytical Chemistry·Yinxi HuangPeng Chen
Mar 25, 2010·Electrophoresis·Wei ChengJonathan M Cooper
Oct 23, 2010·Nanoscale·Tao Li, Wenping Hu
Jan 30, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Inhee ChoiJongheop Yi
Jan 29, 2010·Analytical Chemistry·Tao LiLanqun Mao
Feb 13, 2001·Methods : a Companion to Methods in Enzymology·T P Obrenovitch, E Zilkha

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.