Single cell observations of gas reactions and shape changes in normal and sickling erythrocytes

Biophysical Journal
E AntoniniS Grassi

Abstract

Microspectrophotometry has been applied to single red blood cells to reinvestigate the linked processes of diffusion of gases inside the erythrocyte and their combination with hemoglobin. The experiments took advantage of the photosensitivity of the cabron monoxide derivative of hemoglobin, which allows ligand release from the CO-saturated red cells under strong illumination and recombination when the light is switched off. The photochemical method was also used to study the kinetics of sickling on ligand removal in single erythrocytes of Hb S carriers. The results give new information on the mechanism of the sickling process.

Related Concepts

Anemia, Sickle Cell
Carbon Monoxide
Diffusion
Erythrocytes
Abnormal Red Blood Cell
Photochemistry
Sickle Cell Trait
Spectrophotometry

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