Single-cell omics in ageing: a young and growing field.

Nature Metabolism
Xiaojuan HeGuang-Hui Liu

Abstract

Organismal ageing results from interlinked molecular changes in multiple organs over time. The study of ageing at the molecular level is complicated by varying decay characteristics and kinetics-both between and within organs-driven by intrinsic and extracellular factors. Emerging single-cell omics methods allow for molecular and spatial profiling of cells, and probing of regulatory states and cell-fate determination, thus providing promising tools for unravelling the heterogeneous process of ageing and making it amenable to intervention. These new strategies are enabled by advances in genomic, epigenomic and transcriptomic technologies. Combined with methods for proteome and metabolome analysis, single-cell techniques provide multidimensional, integrated data with unprecedented detail and throughput. Here, we provide an overview of the current state, and perspectives on the future, of this emerging field. We discuss how single-cell approaches can advance understanding of mechanisms underlying organismal ageing and aid in the development of interventions for ageing and ageing-associated diseases.

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Citations

Aug 12, 2020·Protein & Cell·Yingfeng ZhengWenru Su
Sep 12, 2020·Cell Research·Shuai MaGuang-Hui Liu
Oct 30, 2020·Nucleic Acids Research·UNKNOWN Aging Atlas Consortium
Mar 6, 2021·Cell Stem Cell·Cesar A Perez-Ramirez, Heather R Christofk
Mar 18, 2021·Advanced Biology·Carina RamalloGuevaraPavel A Levkin
Apr 4, 2021·Biomolecules·Meytal RadzinskiDana Reichmann
May 31, 2021·Protein & Cell·Hui ZhangGuang-Hui Liu
Jun 29, 2021·Frontiers in Genetics·Serena DatoFrancesco Lescai
Nov 13, 2020·Cell Regeneration·Pengze YanGuang-Hui Liu

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Methods Mentioned

BETA
scRNA-seq
flow
fluorescence-activated cell sorting
single-cell sequencing
amyotrophic lateral sclerosis
Smart-seq
Drop-seq
dissection

Software Mentioned

seq
Drop
seq2
EpiTOF
Smart
Transcriptomics

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