Single-Cell Phenotypic Profiling of CTCs in Whole Blood Using an Integrated Microfluidic Device

Analytical Chemistry
Haimeng PeiBo Tang

Abstract

Single-cell phenotypic profiling of circulating tumor cells (CTCs) in the blood of cancer patients can reveal vital tumor biology information. Even though various approaches have been provided to enrich and detect CTCs, it remains challenging for consecutive CTC sorting, enumeration, and single-cell characterizations. Here, we report an integrated microfluidic device (IMD) for single-cell phenotypic profiling of CTCs that enables automated CTCs sorting from whole blood following continuous single-cell phenotypic analysis while satisfying the requirements of both high purity (92 ± 3%) of cell sorting and high-throughput processing capacity (5 mL whole blood/3 h). Using this new technique we test the phenotypes of individual CTCs collected from xenograft tumor-bearing mice and colorectal (CRC) patients at different tumor stages. We obtained a correlation between CTC characterization and clinical tumor stage and treatment response. The developed IMD offers a high-throughput, convenient, and rapid strategy to study individual CTCs toward minimally invasive cancer therapy prediction and disease monitoring and has the potential to be translated to clinic for liquid biopsy.

References

May 15, 2004·Science·Lotien Richard HuangJames C Sturm
May 3, 2006·Lab on a Chip·David W InglisJames C Sturm
Oct 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shannon L StottMehmet Toner
Mar 15, 2011·Nature·Nicholas NavinMichael Wigler
Mar 26, 2011·Science·Christine L Chaffer, Robert A Weinberg
Jul 4, 2012·Journal of Translational Medicine·David R ParkinsonGary J Kelloff
Apr 5, 2013·Science Translational Medicine·Emre OzkumurMehmet Toner
Sep 14, 2013·Science·Vicki PlaksZena Werb
Jan 22, 2014·Nature Reviews. Clinical Oncology·Matthew G KrebsCaroline Dive
Sep 13, 2014·Lab on a Chip·J McGrathH Bridle
Oct 13, 2015·Nature Biotechnology·Assaf RotemBradley E Bernstein
Dec 9, 2015·Angewandte Chemie·Brenda J GreenShana O Kelley
Feb 11, 2016·Journal of the American Chemical Society·Mahmoud LabibShana O Kelley
Jul 2, 2016·Cell·M Angela NietoJean Paul Thiery
Jan 31, 2017·Journal of the American Chemical Society·Myoung-Hwan ParkPaula T Hammond
Apr 30, 2017·Chemical Science·Yanling SongChaoyong Yang
Sep 22, 2017·Lab on a Chip·Xiaocheng JiangMehmet Toner
Feb 18, 2018·Proceedings of the National Academy of Sciences of the United States of America·Xin HongDaniel A Haber
Sep 19, 2018·Proceedings of the National Academy of Sciences of the United States of America·Manjima DharDino Di Carlo

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Citations

Apr 5, 2020·Mikrochimica Acta·Junjun LuoMinghui Yang
Dec 8, 2020·Analytical Chemistry·Keke HuAndrew G Ewing

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