Single-cell pluripotency regulatory networks

Proteomics
Patrick S StumpfBen D MacArthur

Abstract

Pluripotent stem cells (PSCs) are a popular model system for investigating development, tissue regeneration, and repair. Although much is known about the molecular mechanisms that regulate the balance between self-renewal and lineage commitment in PSCs, the spatiotemporal integration of responsive signaling pathways with core transcriptional regulatory networks are complex and only partially understood. Moreover, measurements made on populations of cells reveal only average properties of the underlying regulatory networks, obscuring their fine detail. Here, we discuss the reconstruction of regulatory networks in individual cells using novel single-cell transcriptomics and proteomics, in order to expand our understanding of the molecular basis of pluripotency, including the role of cell-cell variability within PSC populations, and ways in which networks may be controlled in order to reliably manipulate cell behavior.

References

Nov 14, 2017·Nature Reviews. Genetics·Andrew E Teschendorff, Caroline L Relton
Apr 5, 2017·Scientific Reports·Styliani KontogeorgakiBen D MacArthur

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Related Concepts

Embryo
Biochemical Pathway
Primary Sclerosing Cholangitis
Transcriptional Regulation
Pluripotent Stem Cells
Metabolic Networks and Pathways
Regulation of Biological Process
Reconstructive Surgical Procedures
Transcription, Genetic
Tissue Regeneration

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