Single cell polarity in liquid phase facilitates tumour metastasis

Nature Communications
Anna LorentzenMathias Heikenwalder

Abstract

Dynamic polarisation of tumour cells is essential for metastasis. While the role of polarisation during dedifferentiation and migration is well established, polarisation of metastasising tumour cells during phases of detachment has not been investigated. Here we identify and characterise a type of polarisation maintained by single cells in liquid phase termed single-cell (sc) polarity and investigate its role during metastasis. We demonstrate that sc polarity is an inherent feature of cells from different tumour entities that is observed in circulating tumour cells in patients. Functionally, we propose that the sc pole is directly involved in early attachment, thereby affecting adhesion, transmigration and metastasis. In vivo, the metastatic capacity of cell lines correlates with the extent of sc polarisation. By manipulating sc polarity regulators and by generic depolarisation, we show that sc polarity prior to migration affects transmigration and metastasis in vitro and in vivo.

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Citations

Oct 5, 2018·Chemical Society Reviews·Wen LiXiaogang Qu
Sep 5, 2018·Gut·Jelena MannAriel E Feldstein
Jun 21, 2019·Cellular and Molecular Life Sciences : CMLS·Mathias Heikenwalder, Anna Lorentzen
Dec 22, 2019·Cells·Tsung-Cheng LinHung-Chi Cheng
Aug 22, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xixi WuQinghua Lu
Apr 28, 2019·Nature Reviews. Gastroenterology & Hepatology·Quentin M AnsteeMathias Heikenwalder
Oct 1, 2020·International Journal of Molecular Sciences·Lyubomir HaralambievMatthias B Stope
Apr 26, 2020·Cellular and Molecular Life Sciences : CMLS·Alexios-Fotios A MentisAthanasios G Papavassiliou
May 24, 2020·Annals of Hepatology·Hugo A Barrera-SaldañaSilvia A Barrera-Barrera
Jun 8, 2021·Frontiers in Cell and Developmental Biology·Pan XieZhao-Qian Liu
Jul 31, 2021·Nature Reviews. Cancer·Xin LiMathias Heikenwalder
Dec 10, 2019·ACS Omega·Xixi WuQinghua Lu

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Methods Mentioned

BETA
biopsies
electron microscopy
fluorescence microscopy
xenograft
FCS
xenografts
electrophoresis
FACS
PCR
biopsy

Software Mentioned

tifffile
CellSens Standard
scikits
FACS Diva
Agilent Genomic Feature Extraction
Voloom
Leica LAS
ImageJ
image
Simbiology

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